RLS Determination of Morin Hydrate and Myricetin Using Gold Nanoparticles as Probe
摘 要
利用种子生长法合成金纳米粒子(AuNPs),提出了以AuNPs为共振光散射(RLS)探针测定桑色素和杨梅苷含量的方法。通过静电引力,AuNPs可与桑色素或者杨梅苷结合,形成二元复合物,使RLS强度增强。桑色素/杨梅苷-AuNPs体系的RLS强度增加值ΔIRLS与桑色素/杨梅苷的浓度呈线性关系,线性范围分别为0.40~10.0,0.10~10.0 μmol·L-1,检出限(3S/N)分别为2.98,1.39 μg·L-1。应用该方法测定了合成样品中的桑色素和杨梅苷的含量,加标回收率在97.2%~103%之间,测定值的相对标准偏差(n=5)在0.7%~2.5%之间。
Abstract
Gold nanoparticles (AuNPs) were synthesized by seed growth method, and resonance light scattering (RLS) was proposed for determination of morin hydrate and myricetin using AuNPs as probe. Morin hydrate or myricetin was bond to AuNPs by electrostatic interaction, which leaded to an obviously enhancement of RLS intensity. Linear relationships between ΔIRLS and concentration were obtained in the range of 0.40-10.0 μmol·L-1 for morin hydrate and 0.10-10.0 μmol·L-1 for myricetin, with detection limits (3S/N) of 2.98, 1.39 μg·L-1, respectively. The proposed method was used in the determination of morin hydrate and myricetin in synthesized samples. Test for recovery was made by standard addition method, giving values of recovery in the range of 97.2%-103%, with RSDs (n=5) ranged from 0.7% to 2.5%.
中图分类号 O657.3 DOI 10.11973/lhjy-hx201704003
所属栏目 试验与研究
基金项目 吉林省教育厅“十二五”科技发展计划项目(20140257);长春师范大学研究生教育创新基金项目(cscxy2015002)
收稿日期 2016/6/2
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联系人作者毕淑云(sy_bi@sina.com)
备注周慧凤(1992-),女,内蒙古呼伦贝尔人,硕士研究生,研究方向为光谱分析化学。
引用该论文: ZHOU Hui-feng,WU Jun,WANG Yu,BI Shu-yun. RLS Determination of Morin Hydrate and Myricetin Using Gold Nanoparticles as Probe[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2017, 53(4): 381~386
周慧凤,吴鋆,王瑜,毕淑云. 以金纳米粒子为共振光散射探针测定桑色素和杨梅苷含量[J]. 理化检验-化学分册, 2017, 53(4): 381~386
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参考文献
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【2】宋玉民,康敬万,卢小泉,等.桑色素及其配合物与DNA作用的研究[J].高等学校化学学报, 2003,24(2):249-251.
【3】PATHAK S M, UDUPA N. Pre-clinical evidence of enhanced oral bioavailability of the P-glycoprotein substrate talinolol in combination with morin[J]. Biopharmaceutics & Drug Disposition, 2010,31(2/3):202-214.
【4】赵文华,高军林.荧光光谱法研究桑色素与人血清白蛋白的相互作用[J].福建分析测试, 2013,22(6):10-13.
【5】陈健,郭瑞雪,谢彦瑰.杨梅苷脂质体在动物体内药代吸收动力学初步研究[J].中国食品添加剂, 2010(5):78-82.
【6】喻樊.HPLC法同时测定濒危植物—中华补血草中异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的含量[J].药物分析杂志, 2014,34(4):632-635.
【7】刘焱,高智席,黄成.HPLC-CL法测定桑叶中芦丁和桑色素[J].安徽农业科学, 2008,36(21):9128-9129.
【8】廖声华,田秋霖,吴卫兵.碳糊电极阳极溶出法测定中药甘草中桑色素[J].理化检验-化学分册, 2004,40(3):140-142.
【9】张君才.桑枝中桑色素的双安培法在线测定[J].分析测试学报, 2006,25(1):112-114.
【10】齐洁,万竹青,李默影,等.HPLC法测定杨梅叶中杨梅苷的含量[J].中国野生植物资源, 2012,31(1):35-37.
【11】孟丽娟,程松,潘英妮,等.HPLC法同时测定侧柏叶中杨梅苷等4种黄酮的含量[J].沈阳药科大学学报, 2014,31(2):107-111.
【12】王天娇,毕淑云,赵婷婷,等.CPB与杨梅苷相互作用的共振光散射研究及其应用[J].分子科学学报, 2014,30(1):18-21.
【13】陈展光,丁卫锋,韩雅莉,等.两性离子表面活性剂作为共振光散射探针测定脱氧核糖核酸[J].分析化学, 2005,33(4):519-522.
【14】李原芳,申晓韦,黄承志,等.共振光散射法测定复方氨基酸注射液中色氨酸的含量[J].分析化学, 2008,36(6):819-822.
【15】王雪,吴立航,陈艳华,等.氯金酸共振光散射探针测定阳离子表面活性剂[J].分析化学, 2011,39(12):1882-1886.
【16】BI S Y, WANG Y, ZHOU H F, et al. Assembly of AuNRs and eugenol for trace analysis of eugenol using resonance light scattering technique[J]. Materials Science and Engineering: C, 2016,58:1001-1007.
【17】陶俊.金属纳米颗粒及其聚合物复合薄膜的光学特性研究[D].合肥:中国科学技术大学, 2011.
【18】ROY A S, DINDA A K, CHAUDHURY S, et al. Binding of antioxidant flavonol morin to the native state of bovine serum albumin: effects of urea and metal ions on the binding[J]. Journal of Luminescence, 2014,145:741-751.
【19】BI S Y, WANG T J, ZHAO T T, et al. The resonance Rayleigh light scattering spectral investigation on the interaction of DNA with camellia sinensis in the presence of CPC and its analytical application[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014,127:335-339.
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