CuInS2三元量子点荧光探针测定新霉素
Determination of Neomycin with Ternary CuInS2 Quantum Dots as Fluorescent Probe
摘 要
采用水热法制备了巯基乙酸修饰的CuInS2三元量子点，基于CuInS2量子点荧光强度能够被新霉素显著猝灭的特性，建立了CuInS2三元量子点荧光探针测定新霉素的方法。优化的试验条件如下：① pH 8.0的三羟甲基氨基甲烷-盐酸缓冲溶液的用量为0.5 mL;② CuInS2量子点的浓度为2.0×10-7mol·L-1;③反应时间为5 min。新霉素的浓度在1.0×10-8~2.0×10-7mol·L-1内与其对应的荧光猝灭强度呈线性关系，检出限（3s/k）为2.0×10-10mol·L-1。以空白样品为基体进行加标回收试验，所得回收率在98.4%~106%之间，测定值的相对标准偏差（n=5）在1.9%~2.4%之间。
新霉素
荧光探针
CuInS2 quantum dots
neomycin
fluorescent probe
Abstract
The ternary CuInS2 quantum dots were prepared using thioglycolic acid as modifiers by hydrothermal synthesis method. Based on the fact of the fluorescence intensity of CuInS2 quantum dots could be quenched remarkably by neomycin, a method for determination of neomycin with ternary CuInS2 quantum dots as fluorescent probe was established. The optimized conditions found were as follows:① amount of Tris-HCl buffer solution of pH 8.0:0.5 mL; ② concentration of CuInS2 quantum dots:2.0×10-7mol·L-1; ③ time of reaction:5 min. Linear relationship between values of the fluorescence quenching intensity and concentration of neomycin was obtained in the range of 1.0×10-8-2.0×10-7mol·L-1, with detection limit (3s/k) of 2.0×10-10mol·L-1. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 98.4%-106%, with RSD's (n=5) in the range of 1.9%-2.4%.
中图分类号 O657.3 DOI 10.11973/lhjy-hx201705009
所属栏目 工作简报
基金项目 辽宁省教育厅科学研究项目（L2013138）；辽宁省大学生创新创业训练项目（201410147014）
收稿日期 2016/4/20
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备注毛永强(1974-),男,辽宁昌图人,副教授,博士,主要从事食品安全检测与分析研究
引用该论文: MAO Yongqiang,HU Meina,LI Na. Determination of Neomycin with Ternary CuInS2 Quantum Dots as Fluorescent Probe[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2017, 53(5): 538～541
毛永强,胡美娜,李娜. CuInS2三元量子点荧光探针测定新霉素[J]. 理化检验－化学分册, 2017, 53(5): 538～541
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【3】WANG S, XU B, ZHANG Y, et al. Development of enzyme-linked immunosorbent assay (ELISA) for the detection of neomycin residues in pig muscle, chicken muscle, egg, fish, milk and kidney[J]. Meat Science, 2009,82(1):53-58.
【4】CLAROT I, REGAZZETI A, AUZEIL N, et al. Analysis of neomycin sulfate and framycetin sulfate by high-perfrmance liquid chromatography using evaporative light scattering detection[J]. Journal of Chromatography A, 2005,1087(1/2):236-244.
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【7】WANG J, LIANG J G, SHENG Z G, et al. A novel strategy for selective detection of Ag+ based on the red shift of emission wavelength of quantum dots[J]. Microchimica Acta, 2009,167(3/4):281-287.
【8】LI L, ZHANG F F, DING Y P, et al. Synthesis of functionalized ZnSe nanoparticles and their applications in the determination of bovine serum albumin[J]. Journal of Fluorescence, 2009,19(3):437-441.
【9】QU F, LI H. Selective molecular recognition of polycyclic aromatic hydrocarbons using CdTe quantum dots with cyclodextrin as supramolecular nano-sensitizers in water[J]. Sensors and Actuators B, 2009,135(2):499-505.
【10】LIANG J G, HUANG S, ZENG D Y, et al. CdSe quantum dots as luminescent probes for spironolactone determination[J]. Talanta, 2006,69(1):126-130.
【11】LI D, YAN Z Y, CHENG W Q. Determination of ciprofloxacin with functionalized cadmium sulfide nanoparticles as a fluorescence probe[J]. Spectrochimica Acta Part A, 2008,71(4):1204-1211.
【12】XING H, ZHANG Q X, HUANG X M, et al. Aqueous colloidal CuInS2 for quantum dot sensitized solar cells[J]. Journal of Materials Chemistry, 2011,21(40):15903-15905.
【13】SHEN F, QUE W X, LIAO Y L, et al. Photocatalytic activity of TiO2 nanoparticles sensitized by CuInS2 quantum dots[J]. Industrial and Engineering Chemistry Research, 2011,50(15):9131-9137.
【14】LIU S Y, SHI F P, CHEN L, et al. Tyrosine-functionalized CuInS2 quantum dots as a fluorescence probe for the determination of biothiols, histidine and threonine[J]. Analyst, 2013,138(19):5819-5825.
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