婴幼儿配方食品和成人营养品中脂溶性维生素A和维生素E标准测定方法的比较
Comparison of Standard Methods for Determination of Fat-Soluble Vitamin A and Vitamin E in Infant Formulary Foodstuffs and Adult Nutritives
摘 要
为考核现行的国家标准方法（GB/T 5009.82-2003和GB 5413.9-2010，以下简称GB法）以及国际标准方法AOAC Official Method 2012.10（以下简称AOAC法）在测定婴幼儿配方食品和成人营养品中脂溶性维生素A和维生素E的适用性，对这两种方法进行比对。选择了美国标准物质SRM 1849a、11种婴幼儿配方食品和成人营养品样品分别按上述方法进行分析。结果表明：①从SRM 1849a的分析结果看，两种方法对维生素A和维生素E的测定值均落在认定值的置信界限内；②GB法对维生素E的测定结果偏低，其原因是维生素E较易氧化破坏；③AOAC法预处理操作较简单，工作效率高，有利于在日常检测中应用。
维生素A
维生素E
婴幼儿配方食品
成人营养品
comparison
vitamin A
vitamin E
infant formulary foodstuff
adult nutritive
Abstract
For testing the adaptability of the standard methods of GB/T 5009.82-2003, GB 5413.9-2010 (abbrev. as the GB method) and AOAC Offcial Method 2012.10 (abbrev. as the AOAC method) to the determination of fat-soluble vitamin A (VA) and vitamin E (VE) in infant formulary foodstuffs and adult nutritives, a comparative study on the GB method and AOAC method was made, A CRM of NIST SRM 1849a and 11 infant formulary foodstuffs and adult nutritives samples were chosen to be analyzed separately by the 2 methods. As shown by the experimental results, it was concluded that:① determination results of VA and VE in SRM 1849a found by the GB method and by the AOAC method were all in consistency with the certified values;② determination values of VE found by the GB method was shown to be apparently low due to unstability of VE;③ the AOAC method was found more favourable to be used in routine analysis due to its efficiency and easy operation.
中图分类号 O652.63 DOI 10.11973/lhjy-hx201708007
所属栏目 工作简报
基金项目
收稿日期 2016/8/20
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备注李君绩(1990-),男,山东济南人,硕士研究生,主要从事乳粉(液)中脂溶性维生素A、D和E检测工作
引用该论文: LI Junji,WANG Guoquan,DAI Chengbing,ZHANG Shuang,CHEN Jie,ZHENG Guojian. Comparison of Standard Methods for Determination of Fat-Soluble Vitamin A and Vitamin E in Infant Formulary Foodstuffs and Adult Nutritives[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2017, 53(8): 898～904
李君绩,汪国权,戴承兵,张爽,陈洁,郑国建. 婴幼儿配方食品和成人营养品中脂溶性维生素A和维生素E标准测定方法的比较[J]. 理化检验－化学分册, 2017, 53(8): 898～904
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参考文献
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【24】RAMMELL COLIN G, HOOGENBOOM JACOBUS J L. Separation of tocols by HPLC on an amino-cyano polar phase column[J]. Journal of Liquid Chromatography, 2006,8(4):707-717.
【25】CHÁVEZ-SERVÍN J L, CASTELLOTE A I, LÓPEZ-SABATER M C. Simultaneous analysis of vitamins A and E in infant milk-based formulae by normal-phase high-performance liquid chromatography-diode array detection using a short narrow-bore column[J]. Journal of Chromatography A, 2006,1122(1/2):138-143.
【26】THOMPSON L B, SCHIMPF K, BAUGH S. Determination of vitamins A and E in infant formula and adult/pediatric nutritional formula by HPLC with UV and fluorescence detection:First action 2012.09[J]. Journal of AOAC International, 2013,96(6):1407-1413.
【2】李专成.维生素A合成工艺评述[J].化学工程与装备, 2009(2):95-100.
【3】刘亚军.维生素A缺乏症的预防与治疗[J].中外健康文摘, 2009,6(5):73-74.
【4】宋晓燕,杨天奎.天然维生素E的功能及应用[J].中国油脂, 2000,25(6):45-47.
【5】尤新.天然维生素E的功能和开发前景[J].食品工业科技, 2000,21(4):5-6.
【6】黄挺,张伟,刘军,等.高效液相色谱法-同位素稀释质谱联用法对奶粉中维生素A含量的检测[J].分析测试学报, 2008,27(1):195-196.
【7】张艳海,朱晓艳,曹国洲,等.在线二维柱切换-高效液相色谱法同时测定婴幼儿强化奶粉中维生素A,D3和E的含量[J].分析化学, 2013,41(5):771-775.
【8】黄诚,林胜军.直接皂化-高效液相色谱法测定奶粉中维生素A和E[J].生命科学仪器, 2012,10(2):3-5.
【9】GB/T 5009.82-2003食品中维生素A和维生素E的测定[S].
【10】GB 5413.9-2010婴幼儿食品和乳品中维生素A、D、E的测定[S].
【11】DEVRIES J W, SLIVERA K R, MCSHERRY E, et al. Determination of vitamin A (retinol) in infant formula and adult nutritionals by liquid chromatography:First action 2011.15[J]. Journal of AOAC International, 2012,95(2):322-328.
【12】TURNER C, MATHIASSON L. Determination of vitamins A and E in milk powder using supercritical fluid extraction for sample clean-up[J]. Journal of Chromatography A, 2000,874(2):275-283.
【13】孙亚兰,李帮锐,黎冠,等.反相高效液相色谱法测定奶粉中维生素A和维生素E异构体[J].中国卫生检验杂志, 2013,23(3):612-615.
【14】陈万勤,王瑾,黄丽英,等.高效液相色谱法同时测定配方乳粉中7种脂溶性维生素[J].分析科学学报, 2013,29(1):109-112.
【15】郑菲菲,赖世云,陶保华,等.高效液相色谱法同时测定人乳中视黄醇、α-生育酚和类胡萝卜素[J].食品安全质量检测学报, 2014,5(7):2066-2072.
【16】李颂群,陈胜楠,李征.冷皂化-HPLC法测定乳及乳制品中的维生素[J].中国乳品工业, 2013,41(10):40-42.
【17】钱疆,余孔捷,杨方.液相色谱电化学安培检测乳粉中的脂溶性维生素A,E和D3[J].分析测试技术与仪器, 2006,12(3):176-180.
【18】谢玉珊,林海丹,官咏仪,等.液相色谱法测定婴幼儿配方食品中维生素A和E质量分数[J].中国乳品工业, 2014,42(3):59-61.
【19】甘露,黄明元.液相色谱法同时测定乳制品中的胆固醇和维生素A[J].科技信息, 2010(22):14-15.
【20】王玉堂,池涛,胡本涛,等.婴儿配方粉中维生素A、D、E测定的关键影响因素研究[J].中国食品学报, 2013,13(12):232-236.
【21】张艳海,其布勒哈斯,金燕,等.在线二维液相色谱法同时测定婴幼儿和成人配方营养品中的维生素A、D3和E[J].色谱, 2015,33(3):291-297.
【22】BRABCOVÁ I, KOVÁROVÁ L, ŠATÍNSKý D, et al. A fast HPLC method for determination of vitamin E acetate in dietary supplements using monolithic column[J]. Food Analytical Methods, 2013,6(2):380-385.
【23】GIMENO E, CASTELLOTE A I, LAMUELA-RAVENTOS R M, et al. Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography[J]. Journal of Chromatography A, 2000,881(1/2):251-254.
【24】RAMMELL COLIN G, HOOGENBOOM JACOBUS J L. Separation of tocols by HPLC on an amino-cyano polar phase column[J]. Journal of Liquid Chromatography, 2006,8(4):707-717.
【25】CHÁVEZ-SERVÍN J L, CASTELLOTE A I, LÓPEZ-SABATER M C. Simultaneous analysis of vitamins A and E in infant milk-based formulae by normal-phase high-performance liquid chromatography-diode array detection using a short narrow-bore column[J]. Journal of Chromatography A, 2006,1122(1/2):138-143.
【26】THOMPSON L B, SCHIMPF K, BAUGH S. Determination of vitamins A and E in infant formula and adult/pediatric nutritional formula by HPLC with UV and fluorescence detection:First action 2012.09[J]. Journal of AOAC International, 2013,96(6):1407-1413.
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