采用超高效液相色谱-串联质谱法测定蜂蜜中甲硝唑的含量。蜂蜜样品经磷酸氢二钾溶液溶解，乙酸乙酯提取，用MCS固相萃取柱净化。以Hypersil Gold-C₁₈色谱柱为分离柱，以不同体积比的0.1%（体积分数）甲酸溶液和乙腈混合液为流动相进行梯度洗脱，采用电喷雾正离子源多反应监测模式检测。以D4-甲硝唑为内标物。甲硝唑的线性范围为0.5~64.0 μg·L^-1，方法的测定下限（10S/N）为0.15 μg·kg^-1。以空白样品为基体进行加标回收试验，所得回收率在95.6%~98.8%之间，测定值的相对标准偏差（n=6）在4.2%~8.3%之间。

UHPLC-MS/MS was applied to the determination of metronidazole in honey. The sample of honey was dissolved with di-potassium hydrogen phosphate solution, and then extracted with ethyl acetate. The extract was purified with MCS solid phase extraction column. Hypersil Gold-C₁₈ chromatographic column was used as stationary phase, and the mixture of 0.1% (φ) formic acid solution and acetonitrile mixed in different ratio was used as mobile phase in gradient elution. ESI⁺ and multi-reactions monitor were adopted in MS/MS. D4-metronidazole was used as internal standard. Linearity range of metronidazole was found between 0.5 μg·L^-1 and 64.0 μg·L^-1 with lower limit of determination (10S/N) of 0.15 μg·kg^-1. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 95.6%-98.8%, with RSD's (n=6) in the range of 4.2%-8.3%.