样品0.1000 g经正己烷-二氯甲烷（90+10）混合液溶解并定容至50 mL，经0.45 μm滤膜过滤后，采用正相高效液相色谱法进行分离。以Hypersil NH₂色谱柱为固定相，以不同体积比的正己烷、二氯甲烷和甲醇的混合液为流动相进行梯度洗脱。结果表明：样品无需衍生化，脂肪醇聚氧乙烯醚的组分可按照环氧乙烷（EO）聚合度和碳链得到很好地分离。以自制的C12EO7和C14EO7为标准样品进行定性，面积归一化法定量。按上述方法对AEO-7样品进行分析，其测定值的相对标准偏差（n=6）为2.7%。

The sample (0.100 0 g) was dissolved and made up its volume to 50 mL with a mixture of n-hexane and dichloromethane (90+10). The solution was filtered through 0.45 μm filter membrane, and separated by normal-phase high-performance liquid chromatography using Hypersil NH₂ chromatographic column as stationary phase, and a mixture of n-hexane, dichloromethane and methanol in various ratios as mobile phase in gradient elution. As shown by the results, no derivatization was necessary, and the composition of fatty alcohol ethoxylates (AEO) could be well separated according to the degree of polymerization of ethylene oxide (EO) and the carbon chain. Self-prepared C12EO7 and C14EO7 were used as standard samples for qualitative analysis and the area normalization method was used for quantification. A sample of AEO-7 was analyzed by the above method, giving value of RSD (n=6) of 2.7%.