采用高效液相色谱法同时测定天麻中天麻素、对羟基苯甲醇、对羟基苯甲醛和巴利森苷的含量。样品（1.000 0 g）经60%（体积分数）乙醇40 mL于90℃回流提取1.0 h后进行色谱分离，以Syncronis C₁₈色谱柱为固定相，以不同体积比的甲醇和0.05%（体积分数）磷酸溶液的混合液为流动相进行梯度洗脱。4种化合物的进样质量在一定范围内与其峰面积呈线性关系，检出限（3S/N）在0.003~0.012 μg之间。按标准加入法进行回收试验，回收率在96.4%~98.4%之间，测定值的相对标准偏差（n=6）均小于1.5%。

HPLC was applied to the determination of gastrodin, 4-hydroxybenzyl alcohol, 4-hydroxybenzaldehyde and parishin in Gastrodiaelata. The sample (1.000 0 g) was extracted by reflux at 90℃ for 1.0 h with 40 mL of 60% (φ) ethanol, and then the solution was separated by HPLC using Syncronis C₁₈ column as stationary phase and a mixture of methanol and 0.05% (φ) phosphoric acid solution in various ratios as mobile phase in gradient elution. Linear relationships between values of peak area and injection mass of 4 compounds were found in definite ranges, with detection limits (3S/N) in the range of 0.003-0.012 μg. Test for recovery was made by standard addition method, giving results in the range of 96.4%-98.4%, with RSDs (n=6) all less than 1.5%.