极谱法测定环境水中四环素的含量
Polargraphy Determination of Tetracycline in Environmental Water
摘 要
以十六烷基三甲基溴化铵（CTMAB）对四环素（TC）极谱峰的增敏作用为基础，提出了测定TC含量的极谱法。优化的试验条件如下：CTMAB-Na2HPO4-NaH2PO4缓冲溶液（pH 7.7）为底液，扫描速率0.7 V·s-1，起始电位-0.85 V，静止时间6 s，温度25~30℃。在上述试验条件下，TC的线性范围为0.02~1.0 mg·L-1，1.0~80mg·L-1，检出限（3s/k）为0.001 5 mg·L-1。测定值的相对标准偏差（n=6）均小于4.0%。在3个浓度水平上进行加标回收试验，测得回收率在92.0%~120%之间。
四环素
环境水
十六烷基三甲基溴化铵
缓冲溶液
polarography
tetracycline
environmental water
cetyl trimethyl ammonium bromide
buffer solution
Abstract
A method for determination of tetracycline (TC) by polargraphy was proposed, which was based on the sensitization effect of cetyl trimethyl ammonium bromide (CTMAB) on the polarographic peak of TC. The optimized experimental conditions were as follows:① a mixture of CTMAB and Na2HPO4-NaH2PO4 buffer solution (pH 7.7) was used as the base solution; ② the scanning rate was 0.7 V·s-1; ③ the starting potential was -0.85 V; ④ the stationary time was 6 s; ⑤ the temperature was 25-30℃. In above experimental conditions, linearity ranges for mass concentration of TC found were 0.02-1.0 mg·L-1 and 1.0-80 mg·L-1. Values of detection limit (3s/k) found was 0.001 5 mg·L-1, and values of RSDs (n=6) found were both less than 4.0%. Test for recovery was made by standard addition method at 3 concentration levels, giving results in the range of 92.0%-120%.
中图分类号 O657.14 DOI 10.11973/lhjy-hx201710008
所属栏目 工作简报
基金项目
收稿日期 2016/9/28
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备注孙雪梅(1992-),女,云南曲靖人,硕士研究生,研究方向为环境分析化学(电化学)
引用该论文: SUN Xuemei,GAO Yaling,CHEN Wen,LIU Zhi. Polargraphy Determination of Tetracycline in Environmental Water[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2017, 53(10): 1156～1160
孙雪梅,高娅玲,陈文,刘志. 极谱法测定环境水中四环素的含量[J]. 理化检验－化学分册, 2017, 53(10): 1156～1160
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【11】叶妮,刘智宏,金银珍,等.猪、牛、鱼组织中四环素类药物残留的测定[J].中国兽药杂志, 2006,40(12):5-7.
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