采用固相萃取-高效液相色谱-串联质谱法同时测定不同基质保健食品中10种减肥药物。保健食品样品采用甲醇超声提取，经Oasis HLB固相萃取柱净化，以Zorbax-SB-C₁₈色谱柱为分离柱，以不同体积比的10 mmol·L^-1乙酸铵-0.1%（体积分数）甲酸溶液和乙腈的混合液为流动相进行梯度洗脱，采用电喷雾正离子源多反应监测模式检测。10种减肥药物的质量浓度均在1.0~100.0 μg·L^-1内与其对应的峰面积呈线性关系，方法的检出限（3S/N）为1.2~3.8 μg·kg^-1。以空白样品为基体进行加标回收试验，所得回收率为79.5%~106%，测定值的相对标准偏差（n=6）为2.5%~9.7%。

HPLC-MS/MS was applied to the simultaneous determination of 10 diet drugs in health-care foods of different matrices with solid phase extraction. The health-care food sample was extracted ultrasonically with methanol, and the extract was purified by Oasis HLB solid phase extraction column. Zorbax-SB-C₁₈ chromatographic column was used as stationary phase, and the mixtures of 10 mmol·L^-1 ammonium acetate-0.1% (φ) formic acid solution and acetonitrile mixed in different ratios were used as mobile phase in gradient elution. ESI⁺ and multi-reactions monitor mode were adopted in MS/MS. Linear relationships between values of peak area and mass concentration of the 10 diet drugs were kept in the same range of 1.0-100.0 μg·L^-1, with detection limits (3S/N) in the range of 1.2-3.8 μg·kg^-1. On the base of blank sample, test for recovery was made by standard addition method. Values of recovery found were in the range of 79.5%-106%, with RSD's (n=6) in the range of 2.5%-9.7%.