采用液相色谱-串联质谱法（LG-MS/MS）测定了化妆品（膏霜、乳液或水剂）中15种性激素的含量。样品（0.20 g）与饱和氯化钠溶液（2 mL）混匀分散，用乙腈（每次2 mL）提取2次。合并提取液，加入200 g·L^-1亚铁氰化钾溶液和200 g·L^-1乙酸锌溶液各0.2 mL，混匀使大分子物质沉淀，离心后取上清液，加水定容至10 mL。按不同的程序分别对雌激素、雄激素和孕激素进行梯度洗脱。质谱测定中对雌激素采用负离子模式，雄激素和孕激素采用正离子模式。15种性激素的质量浓度均在8.0~800 μg·L^-1内与其对应的峰面积呈线性关系，方法的检出限（3S/N）为0.05~0.4 mg·kg^-1。经多批实样分析，所得测定值的相对标准偏差（n=6）为0.90%~4.3%，加标回收率为80.3%~121%。

LC-MS/MS was applied to the determination of 15 sex hormones in cosmetics (cream, emulsion or water preparation). The sample (0.20 g) was dispersed in 2 mL of saturated NaCl solution and extracted twice with acetonitrile (2 mL each). 0.2 mL of each of the solution of 200 g·L^-1 K₄Fe(CN)₆ and 200 g·L^-1 (CH₃COO)₂Zn were added to the combined extract to precipitate the large molecular matrix. After centrifugation, the supernatant was taken and its volume was made up to 10 mL. Different programs were used in the gradient elution of estrogen hormones, male hormones and progestin hormones respectively, and in MS/MS analysis, the mode of ESI^- was applied to estrogen hormones and ESI⁺ was applied to male hormones and progestin hormones. Linear relationships between values of peak area and mass concentration of the 15 sex hormones were kept in the same range of 8.0-800 μg·L^-1, with detection limits (3S/N) in the range of 0.05-0.4 mg·kg^-1. Substantial samples of different batches were analyzed by this method, giving values of RSD's (n=6) in the range of 0.90% to 4.3%, and of recovery ranged from 80.3% to 121%.