采用超高效液相色谱-串联质谱法测定葡萄中桔青霉素、链格孢酚和链格孢酚甲基乙醚的残留量。葡萄样品经乙腈提取，氯化钠和无水硫酸镁除水，C₁₈粉末净化除杂，有机滤膜过滤。以ACQUITY UPLC BEH C₁₈色谱柱为分离柱，以不同体积比的乙腈和5 mmol·L^-1乙酸铵溶液的混合液为流动相进行梯度洗脱，采用电喷雾正、负离子源多反应监测模式检测。3种化合物的质量浓度均在5~100 μg·L^-1内与其对应的峰面积呈线性关系，方法的检出限（3S/N）为0.3~0.6 μg·kg^-1。以空白样品为基体进行加标回收试验，所得回收率为79.0%~97.0%，测定值的相对标准偏差（n=5）为0.80%~5.2%。

UHPLC-MS/MS was applied to the determination of residual amounts of citrinin, alternariol and alternariolmethylether in grapes. Grape sample was extracted with acetonitrile, and then NaCl and anhydrous magnesium sulfate were used to remove the water. C₁₈ powder was added for purification in addition to miscellaneous, and organic membrane was used to filter. ACQUITY UPLC BEH C₁₈ chromatographic column was used as stationary phase, and the mixtures of acetonitrile and 5 mmol·L^-1 ammonium acetate solution mixed in different ratios were used as mobile phase in gradient elution. ESI⁺, ESI^- and multi-reactions monitor mode were adopted in MS/MS. Linear relationships between values of peak area and mass concentration of the 3 compounds were kept in the same range of 5-100 μg·L^-1, with detection limits (3S/N) in the range of 0.3-0.6 μg·kg^-1. On the base of blank sample, test for recovery was made by standard addition method. Values of recovery found were in the range of 79.0%-97.0%, with RSD's (n=5) in the range of 0.80%-5.2%.