提出了高效液相色谱-双波长法同时测定不同生长期的黄精中腺苷、人参皂苷Rb₁、β-谷甾醇、香草酸和甘草素等5种活性化学成分含量的方法。样品5.000 0 g经甲醇（70+30）溶液40 mL超声提取30 min，提取液以Diamonsil钻石C₁₈色谱柱为固定相，以0.1%（体积分数）甲酸溶液和0.1%（体积分数）甲酸-乙腈溶液的混合液为流动相进行梯度洗脱，双波长检测（λ₁=252 nm，λ₂=280 nm）。在优化的试验条件下，5种活性化学成分的质量浓度在0.5~100 mg·L^-1范围内与其峰面积呈线性关系，检出限（3S/N）为0.36~0.47 mg·kg^-1。按标准加入法进行回收试验，回收率在96.0%~101%之间，其相对标准偏差（n=6）均小于3.0%。

A method of simultaneous determination of 5 active chemical constituents (adenosine, ginsenoside Rb₁, β-sitosterol, vanillic acid and liquiritigenin) in Polygonatum Sibiricum Red. at different growth stages was proposed by high performance liquid chromatography with dual wavelengths. The sample (5.000 0 g) was extracted ultrasonically for 30 min with 40 mL of methanol (70+30) solution. The extract was separated by HPLC with dual wavelengths (λ₁=252 nm, λ₂=280 nm) using Diamonsil C₁₈ column as the stationary phase, and a mixture of 0.1% (φ) formic acid solution and 0.1% (φ) formic acid-acetonitrile solution in different volume ratios as the mobile phase for gradient elution. Under the optimized test conditions, linear relationships between values of peak area and mass concentration of 5 active chemical constituents were found in the range of 0.5-100 mg·L^-1, with detection limits (3S/N) in the range of 0.36-0.47 mg·kg^-1. Test for recovery was made by standard addition method, giving results in the range of 96.0%-101% and RSDs (n=6) all less than 3.0%.