磁性固相萃取-高效液相色谱法测定葡萄酒中罗丹明B的含量
Magnetic Solid Phase Extraction-HPLC Determination of Rhodamine B in Wine
摘 要
样品溶液（pH 4.0，10 mL）以流量1.0 mL·min-1经自制磁性石墨烯（CoFe2O4-G）20 mg萃取，用1 mL丙酮洗脱。所得溶液中的罗丹明B采用高效液相色谱法进行测定。罗丹明B的质量浓度在0.50~10 μg·L-1内与其峰面积呈线性关系，检出限（3S/N）为0.08 μg·L-1。按标准加入法进行回收试验，回收率为99.0%~101%，相对标准偏差（n=11）为1.6%~4.0%。
高效液相色谱法
CoFe2O4-石墨烯
罗丹明B
葡萄酒
magnetic soild phase extraction
HPLC
CoFe2O4-grapheme
rhodamine B
wine
Abstract
The sample solution (pH 4.0, 10 mL) was extracted with homemade magnetic graphene (CoFe2O4-G) of 20 mg at the flow rate of 1.0 mL·min-1, and acetone of 1 mL was used as the eluent. Rhodamine B in the above solution was determined by HPLC. Linear relationship between peak area and mass concentration of rhodamine B was found in the range of 0.50-10 μg·L-1, with detection limit (3S/N) of 0.08 μg·L-1. Test for recovery was made by standard addition method, giving results in the range of 99.0%-101%, with RSDs (n=11) ranged from 1.6% to 4.0%.
中图分类号 O652.63 DOI 10.11973/lhjy-hx201804007
所属栏目 工作简报
基金项目 江苏省卫生厅预防医学科研基金（Y2015035）；淮安市科技局科技支撑（社会发展）项目（HAS2015030）
收稿日期 2017/4/28
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备注王露,硕士研究生,检验师,主要从事理化检验工作,wlnear@163.com
引用该论文: WANG Lu,XU Rui,WANG Qin,SONG Xin,FENG Xiaoqing,WANG Yi,HANG Xueyu. Magnetic Solid Phase Extraction-HPLC Determination of Rhodamine B in Wine[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2018, 54(4): 403～407
王露,徐瑞,王芹,宋鑫,冯晓青,汪怡,杭学宇. 磁性固相萃取-高效液相色谱法测定葡萄酒中罗丹明B的含量[J]. 理化检验－化学分册, 2018, 54(4): 403～407
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参考文献
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【14】黄亮.固相萃取-液相色谱法测定食品中罗丹明B[J].食品工业, 2011(9):114-116.
【2】POURREZA N, RASTEGARZADEH S, LARKI A. Micelle-mediated cloud point extraction and spectrophotometric determination of rhodamine B using Triton X-100[J]. Talanta, 2008,77(2):733-736.
【3】YU L, MAO Y, QU L. Simple voltammetric determination of rhodamine B by using the glassy carbon electrode in fruit juice and preserved fruit[J]. Food Analytical Methods, 2013,6(6):1665-1670.
【4】ZHANG J, SHAO J, GUO P, et al. A simple and fast Fe3O4 magnetic nanoparticles-based dispersion solid phase extraction of sudan dyes from food and water samples coupled with high-performance liquid chromatography[J]. Analytical Methods, 2013,5(10):2503-2510.
【5】LIU J F, DING J, YUAN B F, et al. Magnetic solid phase extraction coupled with in situ derivatization for the highly sensitive determination of acidic phytohormones in rice leaves by UPLC-MS/MS[J]. Analyst, 2014,139(21):5605-5613.
【6】戚平,周庆琼,林子豪,等.磁性固相萃取-液相色谱法测定环境水样中多种碱性染料[J].华南师范大学学报(自然科学版), 2015,47(2):58-66.
【7】MOAZZEN M, AHMADKHANIHA R, GORJI M E, et al. Magnetic solid-phase extraction based on magnetic multi-walled carbon nanotubes for the determination of polycyclic aromatic hydrocarbons in grilled meat samples[J]. Talanta, 2013,115(17):957-965.
【8】QIANG G, LIN C, DAN L, et al. Magnetic solid-phase extraction using magnetic hypercrosslinked polymer for rapid determination of illegal drugs in urine[J]. Journal of Separation Science, 2011,34(21):3083-3091.
【9】MARCANO D C, KOSYNKIN D V, BERLIN J M, et al. Improved synthesis of graphene oxide[J]. Acs Nano, 2010,4(8):4806-4814.
【10】YAO Y, YANG Z, ZHANG D, et al. Magnetic CoFe2O4-graphene hybrids:Facile synthesis, characterization, and catalytic properties[J]. Industrial & Engineering Chemistry Research, 2012,51(17):6044-6051.
【11】MEROUANI S, HAMDAOUI O, SAOUDI F, et al. Sonochemical degradation of rhodamine B in aqueous phase:Effects of additives[J]. Chemical Engineering Journal, 2010,158(3):550-557.
【12】许家胜,张杰,孙啸虎,等.固相萃取-高效液相色谱法测定饮料中的罗丹明B[J].化学研究与应用, 2014(5):741-743.
【13】李惠茗,李琴,赖祥文,等.石墨烯基固相萃取灵敏检测环境水样中痕量罗丹明B[J].分析试验室, 2015,34(10):1151-1154.
【14】黄亮.固相萃取-液相色谱法测定食品中罗丹明B[J].食品工业, 2011(9):114-116.
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