以抗坏血酸和乙二胺为原料，采用低温回流的方法合成了掺氮碳量子点（N-CDs），盐酸小檗碱对该N-CDs具有明显的荧光猝灭作用，据此提出了以N-CDs为荧光探针测定盐酸小檗碱的方法。在pH 7.40的三羟甲基氨基甲烷-HCl缓冲溶液中，盐酸小檗碱的浓度在5.0×10^-7~4.0×10^-5mol·L^-1内与N-CDs的荧光强度猝灭值呈线性关系，检出限（3s/k）为2.0×10^-7mol·L^-1。方法用于盐酸小檗碱药片的分析，加标回收率为99.2%，103%。通过荧光寿命和吸收光谱的变化以及温度对猝灭常数的影响，确定该N-CDs与盐酸小檗碱的作用机理为动态猝灭。

Nitrogen-doped carbon quantum dots (N-CDs) were synthesized by low temperature reflux method with ascorbic acid and ethylene diamine as raw materials. Berberine hydrochloride could quench the fluorescence of the N-CDs with specificity.Based on the fact,a method was proposed for berberine hydrochloride determination with N-CDs as a fluorescent probe. In Tris-HCl buffer solution of pH 7.40, linear relationship between quenching values of N-CDs fluorescence intensity and concentrations of berberine hydrochloride was kept in the range of 5.0×10^-7-4.0×10^-5mol·L^-1, with detection limit (3s/k) of 2.0×10^-7mol·L^-1. The proposed method was applied to the analysis of tablets of berberine hydrochloride, giving values of recovery of 99.2%, 103%. The dynamic quenching between the N-CDs and berberine hydrochloride was proved by the changes of the fluorescence lifetime as well as the absorption spectrum and the effect of temperature on the quenching constants.