采用固相萃取-超高效液相色谱-串联质谱法测定虾类中4-己基间苯二酚的含量。样品的匀浆（5.00 g）用甲醇提取两次（每次10 mL），合并提取液。移取提取液5 mL，并用水定容至10 mL。此溶液通过PRIME HLB固相萃取柱净化，用含1%（质量分数）氨水的甲醇-乙腈（9+1）溶液4 mL洗脱，洗脱液于40℃水浴中氮气吹至近干，残留物用乙腈（6+4）溶液1 mL溶解。以Waters Acquity UPLC BEH C₁₈色谱柱为分离柱，以不同体积比的水和乙腈的混合液为流动相进行梯度洗脱，采用电喷雾负离子源和多反应监测模式检测。4-己基间苯二酚的质量浓度在1.0~100.0 μg·L^-1内与其对应的峰面积呈线性关系，检出限（3S/N）为0.25 μg·kg^-1。以空白样品为基体进行加标回收试验，所得回收率为70.1%~88.6%，测定值的相对标准偏差（n=6）为3.8%~6.4%。

UHPLC-MS/MS was applied to the determination of 4-hexylresorcinol in shrimps with solid phase extraction. Homogenized pulp (5.00 g) of the sample was extracted twice with methanol (10 mL each). 5 mL of the combined extract was taken,and then made up to 10 mL with water. The solution was purified by passing through PRIME HLB SPE column,and then eluted with 4 mL of mixture of methanol and acetonitrile (9+1) containing 1% (mass fraction) ammonia. The eluate was evaporated to near dryness by N₂-blowing at 40℃ in the water bath.The residue was taken up with 1 mL of acetonitrile (6+4) solution. Waters Acquity UPLC BEH C₁₈ chromatographic column was used as stationary phase, and the mixture of acetonitrile and water mixed in different ratios was used as mobile phase in gradient elution. ESI^- and multi-reactions monitor mode were adopted in MS/MS. Linear relationship between values of peak area and mass concentration of 4-hexylresorcinol was kept in the range of 1.0-100.0 μg·L^-1, with detection limit (3S/N) of 0.25 μg·kg^-1. On the base of blank sample, test for recovery was made by standard addition method. Values of recovery found were in the range of 70.1%-88.6%, with RSD's (n=6) in the range of 3.8%-6.4%.