以索氏提取装置为基础，结合基质固相分散萃取和柱层析的实践，自行设计并制成了集萃取、净化与浓缩于一体的新型样品前处理装置，用于超高效液相色谱法测定吸烟者尿液中苯并[a]芘（B[a]P）和3-羟基苯并[a]芘（3-OHB[a]P）含量时的样品前处理。采集尿样50 mL，加入乙酸盐缓冲溶液（pH 5.0）0.5 g，β-葡糖苷酸-芳基硫酸酯酶0.1 mL，于38℃振荡水解5.0 h。从中分取试液20.00 mL，浓缩至近干，并分散于2.0 g硅胶中。将此分散体烘干后，按规程移入于上述前处理装置中。用60 mL环己烷-乙醇（98+2）混合液于90℃水浴加热回流提取45 min。继续加热蒸发溶液至近干，用1 mL乙腈溶解残留，并过滤后供超高效液相色谱分析。色谱分析中用ACQUITY UPLC^TM BEH C₁₈色谱柱为固定相，以乙腈-水（68+32）混合液为流动相进行洗脱。出峰时间在0~6.5 min时用λ_ex 365 nm和λ_em 450 nm，荧光检测3-OHB[a]P，在6.5~12.0 min时用λ_ex 384 nm和λ_em 406 nm，荧光检测B[a]P，两者的质量浓度依次在4.0~200 μg·L^-1和10~250 μg·L^-1内与相应的峰面积呈线性关系。检出限（3S/N）依次为0.65，3.6 μg·L^-1。加标回收率分别在86.8%~92.8%和91.2%~95.7%之间，测定值的相对标准偏差（n=7）均小于4.0%。

A novel sample pretreatment device integrating extraction, purification and concentration was designed and fabricated on the base of Soxhlet extraction device, and by practice of matrix solid-phase dispersion extraction and column chromatography, and it was used for sample pretreatment in the determination of benzo[a]pyrene (B[a]P) and 3-hydroxybenzo[a]pyrene (3-OHB[a]P) in urine of smokers by ultra high performance liquid chromatography (UPLC). 50 mL of urine sample were collected and hydrolyzed vortically with 0.5 g of acetate buffer solution and 0.1 mL of β-glucuronidearyl sulfatase at 38℃ for 5 h. An aliquot (20.00 mL) of the hydrolyzed solution was concentrated to near dryness and dispersed with 2.0 g of silica gel. The dispersion, after drying, was introduced into the novel sample pretreatment device as prescribed, extracted and purified by refluxing at 90℃ in a water bath for 45 min using 60 mL of a mixture of cyclohexane and ethanol (98+2) as extractant. Heating was continued until the solution was evaporated to near-dryness. The residue was taken up with 1 mL of acetonitrile and the solution, after filtration, was used for UPLC analysis. ACQUITY UPLC^TM BEH C₁₈ column was used as stationary phase and a mixture of acetonitrile and water (68+32) was used as mobile phase to elute the analytes. Fluorophotometric detections were made for 3-OHB[a]P in the interval of peak-appearing time from 0 to 6.5 min at λ_ex 365 nm and λ_em 450 nm, and for B[a]P in the time interval from 6.5 to 12.0 min at λ_ex 384 nm and λ_em 406 nm. Linear relationships between values of peak area and respective mass concentration were found in the range of 4.0 to 200 μg·L^-1 for 3-OHB[a]P and in the range of 10 to 250 μg·L^-1 for B[a]P, with detection limits (3S/N) of 0.65 μg·L^-1 and 3.6 μg·L^-1 respectively. Values of recovery obtained by standard addition method were in the ranges of 86.8% to 92.8% and 91.2% to 95.7% respectively. Values of RSDs (n=7) were all less than 4.0%.