称取经粉碎的样品1.000 0 g，置于加速溶剂提取池中，加入10 mL的5 mol·L^-1盐酸溶液、1 mL的5 mol·L^-1氯化钾溶液和2 mL的10 mol·L^-1半胱氨酸溶液；在8.0 MPa和70℃下，预加热2 min，加热5 min，静态提取5 min，分离，重复上述提取过程2次，合并提取液；加入0.2 mL的0.5 mol·L^-1半胱氨酸溶液、1.5 mL的6 mol·L^-1氢氧化钠溶液，以10 000 r·min^-1离心5 min，将上清液氮吹浓缩并用硝酸（5+95）溶液定容至1 mL，过0.45 μm尼龙滤膜后，采用Eclipse XDB-C₁₈反相色谱柱（150 mm×4.6 mm，5.0 μm）分离溶液中的甲基汞、乙基汞、无机汞、硫柳汞和苯基汞，流动相为50 g·L^-1乙腈溶液+5 g·L^-1乙酸铵溶液+1 g·L^-1半胱氨酸溶液（体积比为1∶1∶1），用原子荧光光谱仪进行测定。5种形态的汞的质量浓度在一定范围内与其荧光强度呈线性关系，测定下限（10S/N）在1.0~2.5 μg·kg^-1之间。加标回收率在91.0%~98.3%之间，测定值的相对标准偏差（n=6）在0.94%~2.7%之间。

The sample (1.000 0 g) was added into the accelerated solvent extraction tank, then 10 mL of 5 mol·L^-1 hydrochloric acid solution, 1 mL of 5 mol·L^-1 potassium chloride solution and 2 mL of 10 mol·L^-1 cysteine solution were added. The mixture was preheated at 8.0 MPa and 70℃ for 2 min, heated for 5 min, extracted statically for 5 min, and seperated. After repeating extraction twice, the extract was combined. Then 0.2 mL of 0.5 mol·L^-1 cysteine solution and 1.5 mL of 6 mol·L^-1 sodium hydroxide solution were added into the extract, and the mixture was centrifuged at 10 000 r·min^-1 for 5 min. The supernatant was concentrated by nitrogen-blowing and the residue was diluted to 1 mL with nitric acid (5+95) solution. After filtration with 0.45 μm nylon membrane, methylmercury, ethylmercury, inorganic mercury, thiomersal and phenylmercury in the solution were separated on an Eclipse XDB-C₁₈ reversed-phase column (150 mm×4.6 mm, 5.0 μm), with a mixture of 50 g·L^-1 acetonitrile solution, 5 g·L^-1 ammonium acetate solution and 1 g·L^-1 cysteine solution (1:1:1, volume ratio) as the mobile phase, and determined by atomic fluorescence spectrometry. Linear relationships were found between the fluorescence intensity and mass concentraion of the five kinds of mercury species in definite ranges, with lower limits of determination (10S/N) in the range of 1.0-2.5 μg·kg^-1. Values of recovery obtained by standard addition method ranged from 91.0% to 98.3%, and RSDs (n=6) ranged from 0.94% to 2.7%.