提出了应用高速逆流色谱从当归挥发油中分离有效成分藁本内酯，探索这一分离条件的关键是两相溶剂体系的选择。根据分离目标物的极性以及选择两相溶剂应满足的3个条件（1分层时间不超过30 s；2对样品不引起分解；3目标成分的分配系数在0.5~2.0之间），经薄层色谱法及高效液相色谱法预选，最终从几种溶剂体系中筛选得到两相溶剂体系为正己烷-乙酸乙酯-乙醇-水（1+1+1+1）体系。采用这一溶剂体系，一次进样500 mg当归挥发油，可达到目标组分与其他杂质完全分离，目标组分回收后得到48 mg藁本内酯。对此分离所得组分经气相色谱-质谱法（GC-MS）分析，其纯度达到98.5%以上，GC-MS还对此组分进行结构鉴定，证明其确为藁本内酯。

High speed countercurrent chromatography (HSCC) was applied to the separation of an effective component of ligustilide from volatile oil of Angelica sinensis. The key to the success of this separation was the choice of the bi-phase solvent system. Based on the polarity of the target component and on the 3 essential requirements (i.e. 1 time of phase separation should be less than 30 s; 2 without leading to decomposition of the sample; 3 values of distribution coefficient K of the targent components should be in the range of 0.5-2.0) which should be followed in the choice of the bi-phase solvent system, the solvent system of n-hexane-ethylacetate-ethanol-water (1+1+1+1) was sieved among some other candidate solvent systems in the preliminary screening by TLC and HPLC. By using the selected bi-phase solvent system in the HSCC separation, when a portion of the oil sample (500 mg) was introduced, 48 mg of ligustilide was obtained after separation. As shown by the results found in the GC-MS study on the separated ligustilide, its purity attained to a high value of more than 98.5%, and through structural study, the compound was identified to be ligustilide.