Preparation of Homocysteine Molecularly Imprinted Polymer Microspheres with Suspension Polymerization and Its Application
摘 要
以同型半胱氨酸(HCY)为模板,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,聚乙烯醇为分散剂,偶氮二异丁腈为引发剂,甲苯为稀释剂,以800 r·min-1转速在55℃水性体系中采用悬浮聚合法制备了HCY分子印迹聚合物微球。制得的分子印迹聚合物微球大小均匀,最佳吸附时间为40 min,最大表观吸附量为1.61 mg·g-1,对结构类似的氨基酸具有良好的选择性。同型半胱氨酸的质量浓度在24 mg·L-1以内与其对应的吸光度呈线性关系,检出限(3s)为0.019 mg·L-1。方法用于家兔血液样品的分析,加标回收率为98.4%~109%,测定值的相对标准偏差(n=5)为0.75%~3.5%。
Abstract
Homocysteine (HCY) molecularly imprinted polymer microspheres were synthesized by suspension polymerization using homocysteine as template, acrylamide as functional monomer, ethylene glycol two methyl acrylate (EGDMA) as crosslinking agent, polyvinyl alcohol (PVA) as dispersant, azobisisobutyronitrile (AIBN) as initiator and toluene as diluent in water system of 55℃ at the speed of 800 r·min-1. It was found that the prepared particles were uniform in size, the optimum adsorption time was 40 min, the maximum apparent adsorption capacity was 1.61 mg·g-1, and the selectivity to other amino acids which structure were similar to HCY was very good. Linear relationship between values of absorbance and mass concentration of homocysteine was obtained within 24 mg·L-1, with detection limit (3s) of 0.019 mg·L-1. The proposed method was applied to the analysis of the sample of rabbit blood, giving values of recovery and RSDs (n=5) in the ranges of 98.4%-109% and 0.75%-3.5% respectively.
中图分类号 O657.32 DOI 10.11973/lhjy-hx201811007
所属栏目 工作简报
基金项目 国家级大学生创新创业基金项目(201611834001);河南省科技厅科技攻关基金项目(172102310419);河南省教育厅自然科学计划基金项目(16B150006);郑州市科技局基金项目(20140789)
收稿日期 2017/11/12
修改稿日期
网络出版日期
作者单位点击查看
备注信建豪,副教授,硕士,主要从事食品、药物分析工作,nilk4@163.com
引用该论文: XIN Jianhao,SHANG Shuyu,WANG Huanhuan,LIU Yingying,HOU Qiaozhi. Preparation of Homocysteine Molecularly Imprinted Polymer Microspheres with Suspension Polymerization and Its Application[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2018, 54(11): 1268~1271
信建豪,尚曙玉,王欢欢,刘颖颖,侯巧芝. 悬浮聚合法制备同型半胱氨酸分子印迹聚合物微球及其应用[J]. 理化检验-化学分册, 2018, 54(11): 1268~1271
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】邵海荣,陈锦春.心脑血管疾病患者血清同型半胱氨酸、血脂检测的临床价值[J].中国农村卫生事业管理, 2017,37(6):739-741.
【2】叶辉,周向京,田耕,等.同型半胱氨酸与心脑血管疾病的关系[J].中国医师杂志, 2006(增刊1):421-422.
【3】刘敬兰,何书美,张宏,等.制药废液中氨基酸的分离与测定[J].理化检验-化学分册, 2008,44(1):76-78.
【4】孙智纯,王兵,单娟娟.种球溶胀悬浮聚合法制备镉(Ⅱ)离子印迹聚合物微球[J].材料研究学报, 2016,30(9):669-674.
【5】丁俊红,张艳,龚树文.辐射引发悬浮聚合制备香草醛分子印迹聚合物[J].辐射研究与辐射工艺学报, 2012,30(1):21-26.
【2】叶辉,周向京,田耕,等.同型半胱氨酸与心脑血管疾病的关系[J].中国医师杂志, 2006(增刊1):421-422.
【3】刘敬兰,何书美,张宏,等.制药废液中氨基酸的分离与测定[J].理化检验-化学分册, 2008,44(1):76-78.
【4】孙智纯,王兵,单娟娟.种球溶胀悬浮聚合法制备镉(Ⅱ)离子印迹聚合物微球[J].材料研究学报, 2016,30(9):669-674.
【5】丁俊红,张艳,龚树文.辐射引发悬浮聚合制备香草醛分子印迹聚合物[J].辐射研究与辐射工艺学报, 2012,30(1):21-26.
相关信息