采用高效液相色谱法测定食用油中特丁基对苯二酚的含量。0.500 0 g食用油样品用乙腈超声萃取两次（每次2 mL），合并萃取液后用流动相稀释至5 mL。以C₁₈色谱柱为分离柱，以乙腈（7+3）溶液为流动相，在检测波长290 nm处进行测定。特丁基对苯二酚的质量浓度在1~150 mg·L^-1内与其对应的峰面积呈线性关系，检出限（3S/N）为0.1 mg·L^-1。方法用于食用油样品的分析，加标回收率为93.5%~98.5%，日内相对标准偏差（n=6）为1.8%，日间相对标准偏差（n=10）为2.9%。

HPLC was applied to the determination of t-butylhydroquinone in edible oil. The edible oil sample (0.500 0 g)was extracted ultrasonically twice with acetonitrile (2 mL each). The combined extract was made up to 5 mL with mobile phase. C₁₈ chromatographic column was used as stationary phase, and a mixture of acetonitrile and water (7+3) was used as mobile phase. Detection at 290 nm was adopted in the determination. Linear relationship between values of peak area and mass concentration of t-butylhydroquinone was kept in the range of 1-150 mg·L^-1, with detection limit (3S/N) of 0.1 mg·L^-1. The proposed method was applied to the analysis of edible oil samples, giving values of recovery in the range of 93.5%-98.5%, intra-day RSD (n=6) of 1.8% and inter-day RSD (n=10) of 2.9%.