采用超高效液相色谱-串联质谱法监测血和尿中吗啡-3-葡萄糖醛酸苷。1.00 mL血样或尿样经3 mL乙腈-甲醇（6+1）混合液提取，离心后，取上清液在40℃水浴中用氮气吹干，残留物用0.2 mL甲醇溶解，经0.22 μm微孔滤膜过滤。以Boston ODS色谱柱为分离柱，以不同体积比的0.1%（体积分数）甲酸溶液和甲醇混合液为流动相进行梯度洗脱，串联质谱分析中采用电喷雾正离子源、多反应监测模式及增强子离子扫描模式。通过保留时间、特征离子对的丰度比对样品进行定性，方法的检出限（3S/N）为5 μg·L^-1。

UHPLC-MS/MS was applied to the detection of morphine-3-glucuronide in blood and urine. The blood or urine sample (1.00 mL) was extracted with 3 mL of the mixture of acetonitrile and methanol (6+1). After centrifugation, the supernatant was evaporated to near dryness by N₂-blowing at 40℃ in the water bath. The residue was taken up with 0.2 mL of methanol. The solution was filtered on 0.22 μm microporous filtering film.Boston ODS chromatographic column was used as stationary phase, and the mixture of 0.1% (φ) formic acid solution and methanol mixed in different ratios was used as mobile phase in gradient elution. ESI⁺, MRM and EPI were adopted in MS/MS. Retention time and characteristic ion pair were used to identify the sample. Detection limit (3S/N) of the method was 5 μg·L^-1.