HPLC Determination of Beta Carotene in Tobacco Leaves with Deep Eutectic Solvent Microextraction
摘 要
采用低共熔溶剂微萃取-高效液相色谱法测定烟叶中β-胡萝卜素的含量。以薄荷醇和月桂酸组成(两者的物质的量之比为2∶1)的低共熔溶剂为萃取剂,低共熔溶剂的用量为400 μL,萃取时间为3.0 min。以Agilent Zorbax SB-C18色谱柱为分离柱,以甲醇-异丙醇(70+30)混合液为流动相,在检测波长448 nm处进行测定。β-胡萝卜素的质量浓度在0.050~9.000 mg·L-1内与其对应的峰面积呈线性关系,检出限(3S/N)为40 μg·L-1。方法用于烟叶样品的分析,加标回收率为70.2%~112%,测定总量的相对标准偏差(n=5)为1.5%~3.7%。
Abstract
HPLC was applied to the determination of beta carotene in tobacco leaves with deep eutectic solvent microextraction. The deep eutectic solvent consisting of menthol and lauric acid (the molar ratio of the two substances was 2:1) was used as extractant. Amount of the deep eutectic solvent was 400 μL and extraction time was 3.0 min. Agilent Zorbax SB-C18 chromatographic column was used as separation column, and the mixture of methanol and isopropanol (70+30) was used as mobile phase. Detection at 448nm was adopted in the determination. Linear relationship between values of peak area and mass concentration of beta carotene was kept in the range of 0.050-9.000 mg·L-1, with detection limit (3S/N) of 40 μg·L-1. The proposed method was applied to the analysis of samples of tobacco leaves, giving values of recovery found by standard addition method and RSDs (n=5) of determined total amounts in the ranges of 70.2%-112% and 1.5%-3.7% respectively.
中图分类号 O652.63 DOI 10.11973/lhjy-hx201908008
所属栏目 工作简报
基金项目 云南中烟工业有限责任公司重点项目(2018GY04)
收稿日期 2018/11/28
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联系人作者汤建国(jgtang@163.com)
备注王程娅,助理工程师,主要从事烟草化学和新型烟草制品技术的研究工作
引用该论文: WANG Chengya,ZHENG Xudong,LEI Ping,HAN Jingmei,LI Zhiqiang,TANG Jianguo,YANG Yaling. HPLC Determination of Beta Carotene in Tobacco Leaves with Deep Eutectic Solvent Microextraction[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2019, 55(8): 912~915
王程娅,郑绪东,雷萍,韩敬美,李志强,汤建国,杨亚玲. 低共熔溶剂微萃取-高效液相色谱法测定烟叶中β-胡萝卜素[J]. 理化检验-化学分册, 2019, 55(8): 912~915
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【5】邵兰军,谢晋,左安建,等.烤后烟叶类胡萝卜素的稳定性及其影响因素[J].烟草科技, 2015,48(7):9-13.
【6】LUO Y G, WANG B J, CUI G M, et al. Effects of different baking processes on carotenoid content in upper leaves of flue-cured tobacco[J]. Agricultural Science & Technology, 2014,15(9):1538-1542.
【7】赵铭钦,刘金霞,黄永成,等.烟叶类胡萝卜素提取最佳条件的研究[J].云南农业大学学报, 2008,23(1):56-59.
【8】FLAKELAR C L, PRENZLER P D, LUCKETT D J, et al. A rapid method for the simultaneous quantification of the major tocopherols, carotenoids, free and esterified sterols in canola (Brassica napus) oil using normal phase liquid chromatography[J]. Food Chemistry, 2017,214:147-155.
【9】LASCU A, BIRDEANU M, TARANU B, et al. Hybrid Mn-porphyrin-nanogold nanomaterial applied for the spectrophotometric detection of β-carotene[J]. Journal of Chemistry, 2018,2018:1-11.
【10】JIMÉNEZ-LPEZ J, SANTOS J L M, ORTEGA-BARRALES P, et al. New perspectives of quantum dots in the food field:Determination of β-carotene in tropical fruit juices and food supplements[J]. Food Analytical Methods, 2017,10(7):2412-2421.
【11】欧阳顺利,周密,曹彪,等.利用二次谐波在体分析番茄中的番茄红素和β-胡萝卜素含量[J].光谱学与光谱分析, 2009,29(12):3362-3364.
【12】刘慧强,陈美婷,王韦达,等.低共熔溶剂萃取超高效液相色谱法检测调味油中酸性橙Ⅱ、碱性橙21和碱性橙22[J].分析试验室, 2018,37(8):932-936.
【13】岳旭东,袁冰,朱国强,等.低共熔溶剂在有机合成和萃取分离中的应用进展[J].化工进展, 2018,37(7):2627-2634.
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