匀浆混匀的豆芽样品经磷酸盐缓冲溶液超声提取，提取液经HLB固相萃取小柱净化，甲醇洗脱。采用超高效液相色谱-串联质谱法测定恩诺沙星和环丙沙星的含量，以Agilent Eclipse Plus C₁₈ RRHD色谱柱为固定相，以不同体积比的甲醇和0.1%（体积分数）甲酸-2 mmol·L^-1乙酸铵溶液的混合液为流动相进行梯度洗脱，串联质谱分析中采用电喷雾正离子源和多反应监测模式。采用同位素内标法定量，恩诺沙星和环丙沙星的线性范围均为0.5~50.0 μg·L^-1，检出限（3S/N）均为0.025 μg·kg^-1。以空白样品为基体进行加标回收试验，所得回收率为89.2%~101%，测定值的相对标准偏差（n=6）为3.3%~9.2%。

The bean sprout sample blended with homogenizer was extracted ultrasonically with phosphate buffer. The extract was purified with HLB solid phase extraction column, and then eluted with methanol. UHPLC-MS/MS was applied to the determination of enrofloxacin and ciprofloxacin. Agilent Eclipse Plus C₁₈ RRHD chromatographic column was used as stationary phase, and the mixture of methanol and 0.1% (φ) formic acid-2 mmol·L^-1 ammonium acetate solution mixed in different ratios was used as mobile phase in gradient elution. ESI⁺ and multi-reactions monitor mode were adopted in MS/MS. Isotope was used as internal standard. Linearity ranges of enrofloxacin and ciprofloxacin were both found between 0.5 μg·L^-1 and 50.0 μg·L^-1, with detection limit (3S/N) of 0.025 μg·kg^-1. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 89.2%-101%, with RSDs (n=6) of determined values in the range of 3.3%-9.2%.