取熟肉样品5.000 g，用乙醇-水（85+15）溶液25.0 mL超声提取15 min，在4℃下快速离心10 min。移取上清液10.0 mL，加入乙醇30 mL，在（60±2）℃下旋转蒸发至近干，用甲醇-水（20+80）溶液5.0 mL分2次溶解残渣，将所得溶液通过全自动固相萃取仪的Oasis HLB SPE柱（500 mg/6 mL），并按程序进行自动净化富集处理，收集洗脱液在40℃下吹氮至近干。用乙醇-水（85+15）溶液2.0 mL溶解残渣，所得溶液经0.22 μm滤膜过滤后作为待测液进行高效液相色谱分析。用Waters SunFire C₁₈色谱柱（250 mm×4.6 mm，5 μm）作为固定相，进样量为20 μL，流动相由甲醇和20 mmol·L^-1乙酸铵溶液按不同比例混合组成，按程序进行3种红曲色素（红曲红胺的检测波长为264 nm，红曲素和红曲红素的检测波长均为390 nm）的分离和测定，采用二极管阵列检测器测定。用3种红曲色素的混合标准溶液系列按方法操作制得3种组分的标准曲线，其相关系数均在0.999 5以上。以3倍信噪比计算得3种组分的检出限（3S/N）依次为0.20，0.060，9.9 mg·kg^-1。以空白样品作为基体，按照标准加入法进行回收试验，测得回收率在92.9%~100%之间，测定值的相对标准偏差（n=6）在1.3%~3.7%之间。

The meat sample (5.000 g) was extracted ultrasonically with 25.0 mL of ethanol-H₂O (85+15) solution for 15 min, and the mixture was centrifuged at 4℃ for 10 min at high speed. The supernatant (10.0 mL) was taken and added with 30 mL of ethanol. The solution was evaporated to near-dryness at (60±2)℃. The residue was taken up with 2 portions of methanol-H₂O (20+80) solution, using 2.5 mL in each dissolution. The analytes of 3 monascus pigments in the methanol solution were then purified and enriched by passing the solution through Oasis HLB SPE column (500 mg/6 mL) of the automatic-SPE installation and operated according to the program automatically. The eluate was collected and evaporated to near-dryness by N₂-blowing at 40℃. The residue was dissolved with 2.0 mL of C₂H₅OH-H₂O (85+15) solution and after filtering through 0.22 μm filtering membrane, the filtrate was used as testing solution for HPLC analysis. Waters SunFire C₁₈ chromatographic column (250 mm×4.6 mm, 5 μm) was used as stationary phase and mixtures with various ratios of methanol and 20 mmol·L^-1 NH₄OAc solution were used as mobile phases in the programed gradient elution for separation of the 3 monascus pigments and their determinations with diode array detector, at wavelengths of 264 nm for monascorubramine and at 390 nm for both monascin and monascorubrin. Linearity ranges between definite mass concentrations of the 3 monascus pigments were obtained having their correlation coeffcients greater than 0.999 5. Detection limits (3S/N) found for the 3 monascus pigments were 0.20, 0.060, 9.9 mg·kg^-1 respectively. Test for recovery was made by standard addition method using a blank sample as matrix, the results obtained were in the range of 92.9%-100%. Values of RSDs (n=6) were found range from 1.3%-3.7%.