吸烟者尿液样品用自主研究开发设计的前处理萃取瓶处理，采用超高效液相色谱-串联质谱法快速测定萃取液中尼古丁和可替宁的含量。以Waters ACQUITY UPLC BEHHILIC色谱柱（50 mm×2.1 mm，1.7 μm）为固定相，以含0.1%（质量分数）氨水的甲醇（6+4）溶液为流动相，串联质谱中采用正离子模式监测。以D₄-尼古丁和D₃-可替宁为内标物，尼古丁和可替宁的线性范围均为20.0~2 000 μg·L^-1，检出限（3S/N）依次为5.7，3.7 μg·L^-1。在2.0，4.0，8.0 μg等3个浓度水平进行加标回收试验，回收率为93.6%~112%，测定值的日内相对标准偏差（n=7）为2.9%~3.4%，日间相对标准偏差（n=7）为3.2%~3.6%。

The urine samples of smokers were prepared by a self-designed extraction vial. UHPLC-MS/MS was applied to the rapid determination of nicotine and cotinine in the extract. Waters ACQUITY UPLC BEHHILIC chromatographic column (50 mm×2.1 mm, 1.7 μm) was used as stationary phase, and methanol (6+4) solution[containing 0.1% (mass fraction) ammonia] was used as mobile phase; positive ionization was used in MS/MS. D₄-nicotine and D₃-cotinine were used as internal standards. Linearity ranges of nicotine and cotinine were found in the same range of 20.0-2 000 μg·L^-1, with detection limits (3S/N) of 5.7,3.7 μg·L^-1, respectively. Tests for recovery were made by standard addition method at the concentration levels of 2.0, 4.0, 8.0 μg. Values of recovery were in the range of 93.6%-112%, and values of intra-day RSDs (n=7) and inter-day RSDs (n=7) of determined values were in the range of 2.9%-3.4% and 3.2%-3.6%, respectively.