应用超高效液相色谱-串联质谱法测定了助眠类保健品中22种禁用安神类药物的含量。将样品连同其包衣和胶囊壳一起研碎后称取1.000 g样品用甲醇溶解，经涡旋混匀并超声15 min，用甲醇定容至25.0 mL。离心后取上清液5.00 mL，经Oasis PRiME HLB固相萃取柱净化处理，收集经净化的流出液，与用于淋洗固相萃取柱的甲醇2 mL合并，于40℃下吹氮至溶液近干。用体积比为1：1的0.05%（体积分数，下同）甲酸-甲醇混合溶液1.00 mL溶解残渣，所得溶液经过0.22 μm滤膜过滤，滤液供色谱分离。选用Poroshell 120 EC-C₁₈色谱柱（50 mm×3.0 mm，2.7 μm）作为固定相，用不同比例的（A）含0.05%甲酸的5 mmol·L^-1乙酸铵溶液和（B）乙腈的混合溶液作为流动相按程序进行梯度洗脱。质谱测定中采用电喷雾离子源和多反应监测模式。结果表明：22种禁用安神类药物的标准曲线中有17种线性范围为4~800 μg·L^-1，有1种为40~8 000 μg·L^-1，还有4种为400~80 000 μg·L^-1，其检出限（3S/N）为0.02~2 μg·g^-1。用标准加入法进行回收试验，测得回收率为81.2%~98.9%，测定值的相对标准偏差（n=6）为3.1%~9.7%。

UPLC-MS/MS was applied to the determination of 22 forbidden sedative-hypnotics in some sleep-aiding health care products. Sample for analysis was prepared by grinding the health care food itself together with its capsula into powder and a portion of 1.000 g was taken and dissolved in methanol with aid of ultrasonic treatment for 15 min. The solution was diluted to 25.0 mL with methanol and centrifuged. An aliquot of 5.00 mL of the supernatant was taken and purified by passing through the Oasis PRiME HLB SPE column. The eluate from the SPE column was collected and mixed with the methanol (2 mL) used in washing the column. The mixed solution was then evaporated to neardryness by N₂-blowing at 40℃. The residue was taken up with 1.00 mL of a mixed solution of 0.005% (volume fraction, same as below) formic acid-methanol with the volume ratio of 1:1, and the solution was filtered through 0.22 μm filtering membrane and the filtrate was used for UPLC separation by passing through Poroshell 120 EC-C₁₈ chromatographic column (50 mm×3.0 mm, 2.7 μm) as stationary phase, and mixture of (A) 5 mmol·L^-1 NH₄OAc solution (containting 0.05% formic acid) and (B) acetonitrile in various ratios were used as mobile phases in gradient elution. In MS/MS determination, ESI and MRM mode were adopted. It was shown that among the standard curves of the 22 forbidden sedative-hypnotics, linearity ranges for 17 sedative-hypnotics were same between 4 μg·L^-1 and 800 μg·L^-1, for one sedative-hypnotic between 40 μg·L^-1 and 8 000 μg·L^-1 and for the remaider 4 sedative-hypnotics between 400 μg·L^-1 and 80 000 μg·L^-1; the detection limits (3S/N) found for these sedative-hypnotics were in the range of 0.02-2 μg·g^-1. Recovery was tested by standard addition method, giving values of recovery in the range of 81.2%-98.9%, and values of RSDs (n=6) in the range of 3.1%-9.7%.