0.100 0 g的甘草提取物样品用10 mL的80%（体积分数）甲醇溶液超声提取30 min后静置30 min，取上清液经0.45 μm滤膜过滤。采用高效液相色谱法同时测定滤液中14种活性成分的含量，以Diamonsil Plus C₁₈色谱柱为分离柱，用0.05%（体积分数）磷酸溶液和乙腈以不同比例混合的溶液为流动相进行梯度洗脱，用二极管阵列检测器测定。通过单因素试验和L9（3³）正交试验对提取条件进行了优化。14种活性成分的质量浓度在一定范围内与其对应的峰面积呈线性关系，方法的检出限（3s）为0.02~0.12 mg·L^-1。在3个浓度水平进行加标回收试验，回收率为91.7%~143%，测定值的相对标准偏差（n=5）为1.2%~6.0%。

The sample (0.100 0 g) of tobacco licorice extract was extracted ultrasonically for 30 min with 10 mL of 80% (φ) methanol solution, and then the above mixture was stood for 30 min. The supernatant was taken and filtered on 0.45 μm filtering membrane. HPLC was applied to the simultaneous determination of 14 active constituents in the filtrate. Diamonsil Plus C₁₈ chromatographic column was used as separated column with mixtures of 0.05% (φ) phosphoric acid solution and acetonitrile with different mixing ratios as mobile phase in the gradient elution, and photodiode array detector was used for determination. The extraction conditions were optimized by single factor tests and L9(3³) orthogonal test. Linear relationships between values of peak area and mass concentration of the 14 active constituents were kept in definite ranges. Detection limits (3s) of the method ranged from 0.02 to 0.12 mg·L^-1. Tests for recovery were made by standard addition method at 3 concentration levels, giving values of recovery and RSDs (n=5) of determination in the ranges of 91.7%-143% and 1.2%-6.0% respectively.