尿液样品经Waters Oasis HLB LP柱萃取，乙腈洗脱，采用高效液相色谱-串联质谱法测定洗脱液中西玛津的含量。以Eclipse Plus C₁₈色谱柱为固定相，以70%（体积分数）乙腈溶液为流动相，串联质谱分析中采用电喷雾离子源正离子模式和多反应监测模式。西玛津的质量浓度在500.0 μg·L^-1以内与其对应的峰面积呈线性关系，检出限（3s/k）为0.91 μg·L^-1，测定下限（10s/k）为3.03 μg·L^-1。以空白样品为基体进行加标回收试验，所得回收率为80.7%~84.0%，测定值的日内相对标准偏差（n=6）和日间相对标准偏差（n=4）分别为1.8%~2.9%，6.8%~9.1%。

The urine sample was extracted with Waters Oasis HLB LP column, and the column was eluted by acetonitrile. HPLC-MS/MS was applied to the determination of simazine in the eluate. Eclipse Plus C₁₈ chromatographic column was used as stationary phase, and 70% (volume fraction) acetonitrile solution was used as mobile phase. ESI⁺ and multi-reactions monitor mode were adopted in MS/MS. Linear relationship between values of peak area and mass concentration of simazine was kept in the range within 500.0 μg·L^-1, with detection limit (3s/k) of 0.91 μg·L^-1 and lower limit of determination (10s/k) of 3.03 μg·L^-1. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 80.7%-84.0%, with intra-day RSDs (n=6) and inter-day RSDs (n=4) of determined values in the ranges of 1.8%-2.9% and 6.8%-9.1%, respectively.