尿液样品离心后经WATERS C₁₈固相萃取柱净化，收集洗脱液。采用二维超高效液相色谱-串联质谱法测定样品溶液中8-羟基脱氧鸟苷的含量。以Waters BEH C₁₈色谱柱（一维色谱）和Waters BEH HILIC色谱柱（二维色谱）为固定相，以0.1%（体积分数，下同）甲酸溶液与乙腈（体积比8：2）组成的混合液（一维色谱）和0.1%甲酸溶液与乙腈（体积比9：1）组成的混合液（二维色谱）为流动相，串联质谱分析中采用电喷雾离子源正离子模式和多反应监测模式。采用内标法定量。8-羟基脱氧鸟苷的线性范围为0.25~300.0 ng·g^-1，检出限（3S/N）为0.10 ng·g^-1。以空白样品为基体进行加标回收试验，所得回收率为96.6%~103%，测定值的日内相对标准偏差（n=6）和日间相对标准偏差（n=4）依次为0.87%~3.3%，1.9%~4.9%。

After centrifugation, the urine sample was purified with WATERS C₁₈ solid phase extraction column, and then the eluate was collected. Two-dimensional UHPLC-MS/MS was applied to the determination of 8-hydroxydeoxyguanosine in the sample solution. Waters BEH C₁₈ chromatographic column (for one-dimensional chromatography) and Waters BEH HILIC chromatographic column (for two-dimensional chromatography) were used as stationary phase. The mixture of 0.1% (φ, the same below) formic acid solution and acetonitrile with volume ratio of 8∶2 (for one-dimensional chromatography) and the mixture of 0.1% formic acid solution and acetonitrile with volume ratio of 9∶1 (for two-dimensional chromatography) were used as mobile phase. ESI⁺ and multi-reactions monitor mode were adopted in MS/MS. Internal standard method was used for quantitative. Linearity range of 8-hydroxydeoxyguanosine was found in the range of 0.25-300.0 ng·g^-1 with detection limit (3S/N) of 0.10 ng·g^-1. On the matrix of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 96.6%-103%, with intra-day RSDs (n=6) and inter-day RSDs (n=4) of determined values in the ranges of 0.87%-3.3% and 1.9%-4.9% respectively.