在采集的尿液样品中依次加入内标和甲醇（控制样品介质中甲醇的体积分数约为50%），所得混合液经自主设计的样品离心分离和过滤装置处理，采用高效液相色谱-串联质谱法快速测定滤液中8-羟基脱氧鸟苷和8-羟基鸟苷的含量。以Synergi Polar-RP色谱柱为固定相，以不同体积比的10 g·L^-1乙酸铵溶液和甲醇的混合液为流动相进行梯度洗脱，串联质谱分析中采用电喷雾离子源正离子模式和多反应监测模式。以¹⁵N₅-8-羟基脱氧鸟苷为内标。8-羟基脱氧鸟苷和8-羟基鸟苷的线性范围均为0.2~40 μg·L^-1，检出限（3S/N）依次为0.054，0.092 μg·L^-1，测定下限（10S/N）依次为0.18，0.32 μg·L^-1。以中度吸烟者的尿液样品为基体进行加标回收试验，所得日内回收率、日间回收率分别为92.3%~96.3%，93.4%~96.3%，测定值的日内相对标准偏差（n=7）和日间相对标准偏差（n=7）分别为1.6%~3.0%，2.8%~3.4%。

The collected urine sample was successively added with internal standard and methanol, and the volume fraction of methanol in the sample medium should be controlled to be about 50%. The above mixture was prepared by self-designed device for centrifugation and filtration of the sample. HPLC-MS/MS was applied to the rapid determination of 8-hydroxydeoxyguanosine and 8-hydroxyguanosine in the filtrate. Synergi Polar-RP chromatographic column was used as stationary phase, and the mixture of 10 g·L^-1 ammonium acetate solution and methanol mixed in different ratios was used as mobile phase in gradient elution. ESI⁺ and multi-reactions monitor mode were adopted in MS/MS. ¹⁵N₅-8-hydroxydeoxyguanosine was used as internal standard. Linearity ranges of 8-hydroxydeoxyguanosine and 8-hydroxyguanosine were found in the same range of 0.2-40 μg·L^-1, with detection limits (3S/N) of 0.054, 0.092 μg·L^-1 and lower limits of determination (10S/N) of 0.18, 0.32 μg·L^-1. On the base of urine sample of a moderate smoker, test for recovery was made by standard addition method; values of intra-day recovery and inter-day recovery were in the ranges of 92.3%-96.3%, 93.4%-96.3% respectively, with intra-day RSDs (n=7) and inter-day RSDs (n=7) of determined values in the ranges of 1.6%-3.0%, 2.8%-3.4% respectively.