护肤类化妆品样品用含0.1 mol·L^-1乙酸铵的95%（体积分数）甲醇溶液提取，提取液经滤膜过滤后，采用液相色谱-串联质谱法测定滤液中5种棕榈酰多肽的含量。以LIChrospher^® 60RP-select B色谱柱为固定相，以不同体积比的含0.3%（体积分数）乙酸的50 mmol·L^-1乙酸铵溶液和乙腈的混合液为流动相进行梯度洗脱，串联质谱分析中采用电喷雾离子源正、负离子模式和选择反应监测模式。5种棕榈酰多肽的质量浓度均在2.0~200.0 μg·L^-1内与其对应的峰面积呈线性关系，测定下限（10S/N）均为2.0 μg·L^-1。以空白样品为基体进行加标回收试验，所得回收率为89.4%~111%，测定值的相对标准偏差（n=6）为1.1%~10%。

The sample of skin-care cosmetic was extracted with 95% (volume fraction) methanol solution containing 0.1 mol·L^-1 ammonium acetate, and then the extract was filtered on filter membrane. LC-MS/MS was applied to the determination of 5 palmitoyl polypeptides in the filtrate. LIChrospher^® 60RP-select B chromatographic column was used as stationary phase, and the mixture of 50 mmol·L^-1 ammonium acetate solution containing 0.3% (volume fraction) acetic acid and acetonitrile mixed in different ratios was used as mobile phase in gradient elution. ESI⁺, ESI^- and selected reaction monitor mode were adopted in MS/MS. Linear relationships between values of peak area and mass concentration of 5 palmitoyl polypeptides were kept in the same range of 2.0-200.0 μg·L^-1, with the same lower limits of determination (10S/N) of 2.0 μg·L^-1. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 89.4%-111%, with RSDs (n=6) of determined values in the range of 1.1%-10%.