建立了超高效液相色谱-串联质谱法（UHPLC-MS/MS）测定化妆品中21种抗组胺类药物的方法。样品用甲醇提取后，采用Waters ACQUITY UPLC BEH-C₁₈色谱柱分离21个组分，以甲醇-含0.1 mol·L^-1甲酸铵的0.1%（体积分数）甲酸溶液为流动相进行梯度洗脱，在电喷雾离子源正离子（ESI⁺）模式下电离，多反应监测（MRM）模式下检测。结果显示：21种抗组胺类药物的质量浓度均在5~250 μg·L^-1内与其对应的峰面积呈线性关系，检出限（3S/N）为0.1~0.5 μg·L^-1。在3种基质中的加标回收率为80.2%~110%，测定值的相对标准偏差（n=6）为1.5%~7.9%。方法用于50批面膜样品的分析，均未检出21种抗组胺类药物。

A method for the determination of 21 antihistamines in cosmetics by UHPLC-MS/MS was established. Samples were extracted with methanol, then 21 components were separated on an Waters ACQUITY UPLC BEH-C₁₈ column with mixed solution composed of methanol and 0.1% (volume fraction) formic acid solution (containing 0.1 mol·L^-1 ammonium formate) as mobile phase for gradient elution. Detection was carried out by ESI⁺ in MRM mode in MS/MS analysis. Linearity relationships between mass concentrations of the 21 antihistamines and their peak areas were kept in the same range of 5-250 μg·L^-1, with detection limits (3S/N) in the range of 0.1-0.5 μg·L^-1. The recoveries on three substrates were found in the range of 80.2%-110%, with RSDs (n=6) of determined values ranged from 1.5% to 7.9%. The proposed method was applied for the analysis of 50 batches of mask samples, and none of 21 antihistamines were found.