建立了高效液相色谱法（HPLC）测定磷脂复合物中硫酸特布他林含量的方法。称取自制的硫酸特布他林磷脂复合物210 mg，用甲醇溶解并定容至10 mL，分取1 mL，再用体积比为35∶65的乙腈-6.5 mmol·L^-1辛烷磺酸钠溶液的混合液（pH 3.4）稀释至10 mL。以AQUASIL^®-C₁₈色谱柱（250 mm×4.6 mm，5 μm）为固定相，以体积比为35∶65的乙腈-6.5 mmol·L^-1辛烷磺酸钠溶液的混合液（pH 3.4）为流动相进行等度洗脱，采用紫外吸收检测器，在280 nm处对硫酸特布他林进行测定，外标法定量。结果显示：硫酸特布他林质量在0.104 3~10.429 0 μg内与其对应的峰面积呈线性关系，检出限（3S/N）为1.565 9 ng；对104.3 mg·L^-1硫酸特布他林对照品溶液重复测定5次，计算得峰面积的相对标准偏差（RSD）为0.18%；对硫酸特布他林复合物进行加标回收试验，回收率为98.5%~100%。方法用于3个硫酸特布他林磷脂复合物样品的测定，复合率为89.04%~91.82%。

A method for the determination of terbutaline sulfate in phospholipid complex by high performance liquid chromatography (HPLC) was established. Self-made terbutaline sulfate phospholipid complex of 210 mg was dissolved with methanol and made its volume up to 100 mL, and then 1 mL of the above solution was diluted with a mixture of acetonitrile and 6.5 mmol·L^-1 sodium octane sulfonate solution at volume ratio of 35∶65 (pH 3.4). AQUASIL^®-C₁₈ column (250 mm×4.6 mm, 5 μm) was used as the stationary phase, and a mixture of acetonitrile and 6.5 mmol·L^-1 sodium octane sulfonate solution at volume ratio of 35∶65 (pH 3.4) was used as the mobile phase for isocratic elution. Terbutaline sulfate was determined by UV absorption detector at 280 nm, and external standard method was used for quantitative analysis. As shown by the results, linear relationship between mass of terbutaline sulfate and its peak area was kept in the range of 0.104 3-10.429 0 μg, with detection limits (3S/N) of 1.565 9 ng. 104.3 mg·L^-1 terbutaline sulfate reference solution was determined 5 times, and RSD of peak area was 0.18%. Recovery test was made on terbutaline sulfate phospholipid complex by standard addition method, giving results in the range of 98.5%-100%. This method was applied for the determination of 3 terbutaline sulfate phospholipid complex samples, and the recombination rate was calculated in the range of 89.04%-91.82%.