建立了高效液相色谱法（HPLC）测定氧化型染发产品中2-氨基-4-羟乙氨基茴香醚及其硫酸盐含量的方法，并采用高效液相色谱-串联质谱法（HPLC-MS/MS）对目标物进行定性确证。称取染发膏0.5 g于比色管中，用体积比为3：7的2.5 g·L^-1 L-半胱氨酸溶液（抗氧化剂）-乙醇的混合液提取并定容至10 mL。以Waters Atlantis^® T3 MV Kit色谱柱为固定相，以含10%（体积分数）乙腈的磷酸盐缓冲溶液-乙腈的混合液为流动相进行梯度洗脱，利用二极管阵列检测器，于304 nm对样品溶液进行测定。HPLC-MS/MS确证分析中采用电喷雾正离子源（ESI⁺）和多反应监测（MRM）模式，当供试品中出现质荷比（m/z）183.0>138.0和m/z 183.0>95.2两个离子对，且离子对间的相对丰度比在允许偏差±20%以内时，可确认供试品中含有2-氨基-4-羟乙氨基茴香醚（硫酸盐）。结果显示：2-氨基-4-羟乙氨基茴香醚的质量浓度在1.984~496.1 mg·L^-1内与其对应的峰面积呈线性关系，检出限（3S/N）为8 μg·g^-1，以2-氨基-4-羟乙氨基茴香醚硫酸盐计，检出限（3S/N）为12 μg·g^-1；不同质量浓度的2-氨基-4-羟乙氨基茴香醚基质匹配标准溶液在24 h内的峰面积的相对标准偏差（RSD，n=7）均小于1.0%；对空白基质样品和染发膏样品进行加标回收试验，回收率为95.5%~101%，测定值的RSD （n=6）为1.9%~2.4%。方法用于7个染发产品分析，其中5个样品中检出2-氨基-4-羟乙氨基茴香醚硫酸盐，检出量为0.053 6%~0.369 0%，与HPLC-MS/MS的确证结果一致；其余2个样品中未检出，与标示内容不相符。

A method for determination of 2-amino-4-hydroxyethylaminoanisole and its sulfate in oxidative hair dye products by high performance liquid chromatography (HPLC) was established, and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used for qualitative verification. 0.5 g of hair dye cream was put into a colorimetric tube and extracted with a mixture of 2.5 g·L^-1 L-cysteine solution (used as the antioxidant) and ethanol at volume ratio of 3:7, and the volume was fixed to 10 mL with the above mixture. Waters Atlantis^® T3 MV Kit chromatographic column was used as stationary phase, and a mixture of the phosphate buffer solution containing 10% acetonitrile and acetonitrile was used as mobile phase for gradient elution. The sample solution was determined at 304 nm by diode array detector. ESI⁺ and MRM mode were used for HPLC-MS/MS analysis verification. When ion pairs of m/z 183.0>138.0 and m/z 183.0>95.2 were appeared in tested sample, and the deviation of the relative abundance ratio of ion pairs was within ±20%, 2-amino-4-hydroxyethylaminoanisole (sulfate) was confirmed in the tested sample. As shown by the results, linear relationship between mass concentration of 2-amino-4-hydroxyethylaminoanisole and its peak area was kept in the range of 1.984-496.1 mg·L^-1, with detection limits of 8 μg·g^-1 for 2-amino-4-hydroxyethylaminoanisole and 12 μg·g^-1 for 2-amino-4-hydroxyethylaminoanisole sulfate. RSDs (n=7) of peak areas of 2-amino-4-hydroxyethylaminoanisole matrix matching standard solutions with different mass concentrations in 24 h were less than 1.0%. Recovery test was made on blank matrix sample and hair dye sample by standard addition method, giving results in the range of 95.5%-101%, and RSDs (n=6) of the determined values were ranged from 1.9% to 2.4%. This method has been applied for analysis of 7 hair dye products. 2-Amino-4-hydroxyethylaminoanisoleand sulfate was detected in 5 samples, with the detection amounts in the range of 0.053 6%-0.369 0%, which was consistent with HPLC-MS/MS verification results, while it was not detected in the other 2 samples, which was inconsistent with the labeling content.