建立了分散液相微萃取-高效液相色谱法（HPLC）测定婴儿血清中美罗培南含量的方法。将经美罗培南药物治疗24 h后的婴儿全血离心，移取婴儿血清样品100 μL，加入50 mg·L^-1甲硝唑（内标）溶液50 μL和乙腈100 μL，涡旋，离心，取全部上清液，加入二氯甲烷100 μL，再次涡旋，离心，取上清液供HPLC分析。以Welch C₁₈色谱柱为固定相，以体积比为1：9的3-吗啡丙磺酸缓冲液（pH 7.0）-乙腈混合液为流动相进行等度洗脱。采用基质匹配混合标准溶液系列制作工作曲线，内标法定量。结果显示：美罗培南工作曲线的线性范围为0.25~100 mg·L^-1，检出限为0.06 mg·L^-1；对空白血清样品进行3个浓度水平的加标回收试验，回收率为98.6%~99.9%，测定值的相对标准偏差（RSD，n=6）为1.2%~2.0%。方法用于15名婴儿患者血清分析，美罗培南的检出量为19.2~98.9 mg·L^-1。

A method for determination of meropenem in infant serum by high performance liquid chromatography (HPLC) coupled with disperse liquid-liquid microextraction was established. 50 μL of 50 mg·L^-1 metronidazole (internal standard) solution and 100 μL of acetonitrile were added into 100 μL of infant serum sample from whole blood of the infant treated with moropenem for 24 h after centrifugation, and 10 μL of dichloromethane was added into all the supernatant after vortex and centrifugation. Then supernatant was taken for HPLC analysis after vortex and centrifugation. Welch C₁₈ column was used as the stationary phase, and a mixture of 3-morphine propyl acid buffer (pH 7.0) and acetonitrile at volume ratio of 1:9 was used as the mobile phase for isocratic elution. Working curve was made by matrix matching mixed standard solution series, and internal standard method was used for quantitative analysis. As shown by the results, linear range of meropenem working curve was in the range of 0.25-100 mg·L^-1, with detection limit of 0.06 mg·L^-1. Recovery test was made on blank serum sample at 3 concentration levels by standard addition method, giving results in the range of 98.6%-99.9%, and RSDs (n=6) of the determined values were ranged from 1.2% to 2.0%. This method has been applied for analysis of 15 actual infant serum samples, with detection amounts of meropenem in the range of 19.2-98.9 mg·L^-1.