Screening of 203 Pesticide Residues in Hair Loss Prevention Cosmetic Containing Plant Extracts by UHPLC-Q-TOF-MS
摘 要
采用超高效液相色谱-四极杆飞行时间质谱法(UHPLC-Q-TOF-MS)构建了203种农药的质谱数据库,将该数据库用于植物类防脱发化妆品中农药残留的筛查。203种农药单标准溶液经UHPLC-Q-TOF-MS分析后,将获得的各农药母离子和所有子离子质荷比(m/z)及丰度比等质谱数据和保留时间导入UNIFI软件中,得到203种农药数据库。24批液体类化妆品经甲醇提取,离心后,经0.22μm微孔滤膜过滤。以10mmol·L-1乙酸铵-乙酸溶液(pH5.0)为水-甲醇流动相的添加剂,以电喷雾离子源正、负离子模式电离,以全信息串联质谱模式采集样品的质谱数据,至少检测1个母离子和1个子离子。将得到的质谱数据与数据库中各农药的保留时间、母离子和子离子m/z及丰度比进行匹配,当满足保留时间绝对偏差的绝对值在0.2min内,母离子和子离子m/z相对偏差的绝对值小于5×10-6,响应强度大于500时,确证样品中含有该农药。结果显示,24批样品中有1批样品检出嘧菌酯。
Abstract
A mass spectrometry database of 203 pesticides was established by ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF-MS), which was used for the screening of pesticide residues in hair loss prevention cosmetic containing plant extracts. The single standard solutions of 203 pesticides were analyzed by UHPLC-Q-TOF-MS, and the obtained MS information containing mass-to-charge ratio (m/z) of the precursor and product ions and their abundance ratio, as well as retention time of each pesticide were imported into UNIFI software to establish the database of 203 pesticides. The 24 batches of liquid cosmetics were extracted with methanol. After centrifugation, the solution was filtered by 0.22 μm microporous membrane. The mobile phase containing water and methanol was supplemented 10 mmol·L-1 ammonium acetate-acetic acid solution (pH 5.0), and electrospray ionization source with positive and negative modes was used for ionization. MS data of the samples were collected with data-independent acquisition mass spectrometry mode, and at least one precursor ion and one product ion were detected. The MS data obtained were matched with the retention time, m/z and abundance ratios of the precursor and product ions of pesticide in the database. When the absolute value of the absolute deviation of retention time was within 0.2 min, the absolute values of the relative deriations of m/z of precursor and product ions were less 5×10-6, and the intensity response was greater than 500, it was indicated that the pesticide was confirmed in the sample. The results showed that azoxystrobin was detected in one from 24 batches of the samples.
中图分类号 O657.63 DOI 10.11973/lhjy-hx202301005
所属栏目 工作简报
基金项目
收稿日期 2022/3/15
修改稿日期
网络出版日期
作者单位点击查看
备注董亚蕾,副研究员,博士,研究方向为化妆品安全检测新方法和新技术
引用该论文: DONG Yalei,QIAO Yasen,NIU Shuijiao,WANG Haiyan,SUN Lei,LU Yong. Screening of 203 Pesticide Residues in Hair Loss Prevention Cosmetic Containing Plant Extracts by UHPLC-Q-TOF-MS[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2023, 59(1): 34~43
董亚蕾,乔亚森,牛水蛟,王海燕,孙磊,路勇. 超高效液相色谱-四极杆飞行时间质谱法筛查植物类防脱发化妆品中203种农药残留[J]. 理化检验-化学分册, 2023, 59(1): 34~43
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】THIBANE V S, NDHLALA A R, ABDELGADIR H A, et al. The cosmetic potential of plants from the Eastern Cape Province traditionally used for skincare and beauty[J]. South African Journal of Botany, 2019,122:475-483.
【2】CHIOCCHIO I, MANDRONE M, SANNA C, et al. Screening of a hundred plant extracts as tyrosinase and elastase inhibitors, two enzymatic targets of cosmetic interest[J]. Industrial Crops and Products, 2018,122:498-505.
【3】王爱娟.中药育发祛屑洗剂治疗雄激素性秃发的临床和实验研究[D].北京:北京中医药大学, 2019.
【4】贺行理商,黄家辉,陈奕公,等.基于社会网络分析法的国产中药育发类化妆品的现状分析[J].中国现代应用药学, 2020,37(12):1409-1416.
【5】周伟璐,孔维军,杨美华,等.中药化妆品中原料药的潜在毒副作用评析[J].中草药, 2016,47(2):352-357.
【6】庞国芳,常巧英,范春林.农药残留监测技术研究与监控体系构建展望[J].中国科学院院刊, 2017,32(10):1083-1090.
【7】孙新琪,安芳,鹿倩,等.我国中药材禁用农药残留现状、毒性及分析方法研究进展[J].中国中药杂志, 2022,47(3):611-627.
【8】付衍宽,孙志洪.加速溶剂萃取-凝胶渗透色谱法净化-气相色谱-串联质谱法测定土壤中常见的7种农药残留量[J].理化检验-化学分册, 2021,57(8):688-692.
【9】杜秋瑶,张云峰,王继芬,等.超高效液相色谱-四极杆-飞行时间质谱法定性筛查和定量分析血液中110种农药[J].理化检验-化学分册, 2020,56(8):851-864.
【10】周相娟,赵玉琪,许华.气相色谱-质谱法测定化妆品中16种有机氯农药[J].分析试验室, 2013,32(12):74-78.
【11】胡守江,杨晋青,顾宇翔.气相色谱-质谱法测定含植物提取物类化妆品中35种农药残留[J].香料香精化妆品, 2021(5):83-91.
【12】周建峰,隋玉杰,张碧宇,等.含植物提取物类化妆品中7种禁用农药残留量UPLC-MS/MS方法的研究[J].农产品加工, 2021(9):63-67.
【13】MENG X S, BAI H, GUO T, et al. Broad screening of illicit ingredients in cosmetics using ultra-high-performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometry with customized accurate-mass database and mass spectral library[J]. Journal of Chromatography A, 2017,1528:61-74.
【14】席绍峰,李慧勇,谭建华,等.SPE-UPLC-Q-TOF MS测定育发化妆品中人参和甘草类功效成分[J].质谱学报, 2015,36(4):341-349.
【15】董亚蕾,牛水蛟,乔亚森,等.超高效液相色谱-四极杆-飞行时间质谱法测定防脱发化妆品中19种非法添加化学成分[J].色谱, 2022,40(4):343-353.
【2】CHIOCCHIO I, MANDRONE M, SANNA C, et al. Screening of a hundred plant extracts as tyrosinase and elastase inhibitors, two enzymatic targets of cosmetic interest[J]. Industrial Crops and Products, 2018,122:498-505.
【3】王爱娟.中药育发祛屑洗剂治疗雄激素性秃发的临床和实验研究[D].北京:北京中医药大学, 2019.
【4】贺行理商,黄家辉,陈奕公,等.基于社会网络分析法的国产中药育发类化妆品的现状分析[J].中国现代应用药学, 2020,37(12):1409-1416.
【5】周伟璐,孔维军,杨美华,等.中药化妆品中原料药的潜在毒副作用评析[J].中草药, 2016,47(2):352-357.
【6】庞国芳,常巧英,范春林.农药残留监测技术研究与监控体系构建展望[J].中国科学院院刊, 2017,32(10):1083-1090.
【7】孙新琪,安芳,鹿倩,等.我国中药材禁用农药残留现状、毒性及分析方法研究进展[J].中国中药杂志, 2022,47(3):611-627.
【8】付衍宽,孙志洪.加速溶剂萃取-凝胶渗透色谱法净化-气相色谱-串联质谱法测定土壤中常见的7种农药残留量[J].理化检验-化学分册, 2021,57(8):688-692.
【9】杜秋瑶,张云峰,王继芬,等.超高效液相色谱-四极杆-飞行时间质谱法定性筛查和定量分析血液中110种农药[J].理化检验-化学分册, 2020,56(8):851-864.
【10】周相娟,赵玉琪,许华.气相色谱-质谱法测定化妆品中16种有机氯农药[J].分析试验室, 2013,32(12):74-78.
【11】胡守江,杨晋青,顾宇翔.气相色谱-质谱法测定含植物提取物类化妆品中35种农药残留[J].香料香精化妆品, 2021(5):83-91.
【12】周建峰,隋玉杰,张碧宇,等.含植物提取物类化妆品中7种禁用农药残留量UPLC-MS/MS方法的研究[J].农产品加工, 2021(9):63-67.
【13】MENG X S, BAI H, GUO T, et al. Broad screening of illicit ingredients in cosmetics using ultra-high-performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometry with customized accurate-mass database and mass spectral library[J]. Journal of Chromatography A, 2017,1528:61-74.
【14】席绍峰,李慧勇,谭建华,等.SPE-UPLC-Q-TOF MS测定育发化妆品中人参和甘草类功效成分[J].质谱学报, 2015,36(4):341-349.
【15】董亚蕾,牛水蛟,乔亚森,等.超高效液相色谱-四极杆-飞行时间质谱法测定防脱发化妆品中19种非法添加化学成分[J].色谱, 2022,40(4):343-353.
相关信息