体外皮肤变态反应-氨基酸衍生物反应试验在防晒剂致敏性初筛中的应用
Application of In Vitro Skin Allergy-Amino Acid Derivative Reactivity Assay in Preliminary Screening of Sensitization of Sunscreens
摘 要
采用氨基酸衍生物反应试验(ADRA)对20种化学防晒剂的致敏性进行评价。利用半胱氨酸衍生物N-[2-(1-萘基)乙酰基]-L-半胱氨酸(NAC)和赖氨酸衍生物α-N-[2-(1-萘基)乙酰基]-L-赖氨酸(NAL)模拟皮肤蛋白,与化学防晒剂共同反应,通过高效液相色谱法测定20种化学防晒剂的氨基酸衍生物消耗百分比,并对皮肤致敏性进行预测。通过比较ADRA预测结果与欧盟消费者安全科学委员会(SCCS)出示的化合物评估报告的一致性,判断方法的可行性。结果表明,3-亚苄基樟脑等19种化学防晒剂预测结果为阴性,二苯酮-3预测结果为阳性。ADRA可作为动物试验体外替代方法,简便、快捷地对化合物的皮肤致敏性进行预测和筛选。
高效液相色谱法
皮肤致敏性
化学防晒剂
amino acid derivative reactivity assay (ADRA)
high performance liquid chromatography
skin sensitization
chemical sunscreen
Abstract
The sensitization of 20 chemical sunscreens was evaluated using amino acid derivative reactivity assay (ADRA). Cysteine derivative N-[2-(1-naphthyl) acetyl]-L-cysteine (NAC) and lysine derivative α-N-[2-(1-naphthyl) acetyl]-L-lysine (NAL) were used to mimic skin proteins, which reacted with chemical sunscreens. The amino acid derivative depletion percentage of 20 chemical sunscreens were determined by high performance liquid chromatography, and then their skin sensitization were predicted. The feasibility of ADRA method was judged by comparing the consistency of ADRA prediction results with the compound evaluation reports produced by European Scientific Committee on Consumer Safety (SCCS). It was shown that 19 chemical sunscreens such as 3-benzylidene camphor were predicted to be negative, and benzophenone-3 was predicted to be positive. ADRA could be used as an alternative in vitro method for animal experiments to predict and screen the skin sensitization of compounds simply and quickly.
中图分类号 O657.7 DOI 10.11973/lhjy-hx202306005
所属栏目 工作简报
基金项目 中国药品监管科学行动计划重点项目;国家药品监督管理局化妆品标准研究(2021-BZ-014)
收稿日期 2022/4/14
修改稿日期
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联系人作者于海英(myhy12@163.com)
备注张良雨,硕士,主要从事化妆品、保健食品理化检验与研究工作
引用该论文: ZHANG Liangyu,LIN Yujia,JIANG Yaqi,LI Qiyan,YU Haiying. Application of In Vitro Skin Allergy-Amino Acid Derivative Reactivity Assay in Preliminary Screening of Sensitization of Sunscreens[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2023, 59(6): 649～655
张良雨,林钰镓,蒋亚奇,李启艳,于海英. 体外皮肤变态反应-氨基酸衍生物反应试验在防晒剂致敏性初筛中的应用[J]. 理化检验－化学分册, 2023, 59(6): 649～655
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【3】喻才正,周婷,黄倩,等.动物皮肤致敏试验方法的研究进展[J].华中科技大学学报(医学版), 2016,45(2):224-228.
【4】Organization for Economic Cooperation and Development. In vitro skin sensitisation: ARE-Nrf2 luciferase test method: TG 442D[S]. Paris: Organization for Economic Cooperation and Development, 2015.
【5】COTTREZ F, BOITEL E, AURIAULT C, et al. Genes specifically modulated in sensitized skins allow the detection of sensitizers in a reconstructed human skin model. Development of the SENS-IS assay[J]. Toxicology in Vitro, 2015,29(4):787-802.
【6】李晨,沙毅杰,霍倩,等.人源THP-1细胞系活化试验在化妆品皮肤致敏性评价中的应用研究[J].现代预防医学, 2022,49(2):349-353.
【7】HUPPERT C, PARIS C, LANGONNÉ I, et al. Activation of T cells by dendritic cells exposed to a reference sensitizer: Towards a promising model to assess the allergenic potential of chemicals[J]. Contact Dermatitis, 2018,79(2):67-75.
【8】Organization for Economic Cooperation and Development. In chemico skin sensitisation: Assays addressing the adverse outcome pathway key event on covalent binding to proteins: TG 442C[S]. Paris: Organization for Economic Cooperation and Development, 2019.
【9】耿雪,王小兵,魏霞,等.一种新型in silico模型在化妆品原料皮肤致敏性初筛中的应用[J].香料香精化妆品, 2020(2):27-32.
【10】YAMAMOTO Y, TAHARA H, USAMI R, et al. A novel in chemico method to detect skin sensitizers in highly diluted reaction conditions[J]. Journal of Applied Toxicology, 2015,35(11):1348-1360.
【11】所雅琼,罗飞亚,张凤兰,等.皮肤致敏替代方法和整合策略研究进展及思考[J].香料香精化妆品, 2021(5):96-102.
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