Analysis of 23 Elements in Liuwei Dihuang Pills by Inductively Coupled Plasma Mass Spectrometry with Chemometrics
摘 要
提出了电感耦合等离子体质谱法(ICP-MS)同时测定六味地黄丸中钠、镁、铝、钾、钙、钒、铬、锰、铁、钴、镍、铜、锌、镓、砷、硒、铷、锶、镉、铯、钡、铊、铅等23种元素的含量,结合化学计量学对各元素含量进行分析,并根据人用药品注册技术要求国际协调会颁布的元素杂质指导原则对镉、铅、砷、钴、钒、镍、铜、铬等8种有害元素进行健康风险评估。六味地黄丸通过微波消解法处理,碰撞池模式下选用45Sc作为内标测定23Na、24Mg、27Al、39K、40Ca、51V、52Cr、55Mn、56Fe的含量,73Ge作为内标测定59Co、59Ni、64Cu、66Zn、70Ga、75As、79Se、85Rb、88Sr的含量,115In作为内标测定112Cd、133Cs、137Ba的含量,209Bi作为内标测定205Tl、207Pb的含量。结果显示:23种元素的质量浓度在一定范围内与对应的各元素响应值与内标响应值比值呈线性关系,检出限(3s/k)为0.000 9~4.807 μg·L-1;按照标准加入法进行回收试验,加标回收率为84.9%~112%。根据21批样品测定结果,建立六味地黄丸的元素指纹图谱,利用SPSS 20.0软件进行主成分分析和聚类分析,并对有害元素进行健康风险评估。结果表明:六味地黄丸中23种元素含量具有一定规律,指纹图谱可用于鉴别六味地黄丸;主成分分析得到4个主成分,累计方差贡献率为81.579%,筛选出钒、钴、铯、铁、铊、镓、钡、镁、锶、钠、硒、镉为六味地黄丸的特征元素;聚类分析将21批样品分为3类,大多数样品中元素含量具有相似性;健康风险评估结果显示其中一批样品中铅元素含量存在健康风险,其余批次样品中有害元素健康风险在可接受范围内。
Abstract
A method for the simultaneous determination of 23 elements, including Na, Mg, Al, K, Ca, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Se, Rb, Sr, Cd, Cs, Ba, Tl and Pb in Liuwei Dihuang pills by inductively coupled plasma mass spectrometry (ICP-MS) was proposed, and the content of each element was analyzed with chemometrics. According to guideline for elemental impurities issued by International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use, health risks of 8 harmful elements including Cd, Pb, As, Co, V, Ni, Cu and Cr were evaluated. The Liuwei Dihuang pills were digested by microwave, and 23Na, 24Mg, 27Al, 39K, 40Ca, 51V, 52Cr, 55Mn and 56Fe were determined by using 45Sc as internal standard with collision pool mode, 59Co, 59Ni, 64Cu, 66Zn, 70Ga, 75As, 79Se, 85Rb and 88Sr by using 73Ge as internal standard, 112Cd, 133Cs and 137Ba by using 115In as internal standard, 205Tl and 207Pb by using 209Bi as internal standard. It was shown that the linear relationships between the mass concentrations of 23 elements and the ratios of corresponding response values of each element to internal standard in definite ranges, with detection limits (3s/k) in the range of 0.000 9-4.807 μg·L-1. Test for recovery was made by standard addition method, giving results in the range of 84.9%-112%. The fingerprints of elements of Liuwei Dihuang pills were established according to the determination results of 21 batches of samples, and SPSS 20.0 software was used for principal component analysis and cluster analysis, and the health risk assessment of harmful elements was conducted. The results showed that the contents of 23 elements in Liuwei Dihuang pills had certain regularity, and the fingerprints could be used to identify Liuwei Dihuang pills. Four principal components were obtained by principal component analysis, and the cumulative variance contribution rate was 81.579%, and V, Co, Cs, Fe, Tl, Ga, Ba, Mg, Sr, Na, Se and Cd were selected as the characteristic elements of Liuwei Dihuang pills. The 21 batches of samples were divided into 3 categories by cluster analysis, and the contents of elements in most samples were similar. The health risk assessment showed that the content of Pb in one batch of sample presented a health risk, while the health risk of harmful elements in the remaining batch of samples was within the acceptable range.
中图分类号 O657.63 DOI 10.11973/lhjy-hx202307009
所属栏目 专题报道(化学计量学方法在食品药品分析中的应用)
基金项目 重庆市技术创新与应用发展专项面上项目(cstc2020jscx-msxmX0081);重庆市教委科技项目(KJQN202102809)
收稿日期 2022/6/27
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备注代磊,硕士研究生,研究方向为药物分析
引用该论文: DAI Lei,LI Yan,HOU Yuanfang,HE Dan,YANG Lin. Analysis of 23 Elements in Liuwei Dihuang Pills by Inductively Coupled Plasma Mass Spectrometry with Chemometrics[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2023, 59(7): 798~805
代磊,李艳,侯媛芳,何丹,杨林. 电感耦合等离子体质谱法结合化学计量学分析六味地黄丸中23种元素[J]. 理化检验-化学分册, 2023, 59(7): 798~805
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【8】CANNAS D, LOI E, SERRA M, et al. Relevance of essential trace elements in nutrition and drinking water for human health and autoimmune disease risk[J]. Nutrients, 2020,12(7):2074.
【9】杨殷凯,王琪瑞,孙思雅,等.浙江道地药材的质量影响因素和品质评价技术的现状与展望[J].浙江农业科学, 2020,61(6):1039-1043.
【10】徐芳琴,张新睿,郭凤伟,等.中成药质量控制研究进展[J].现代生物医学进展, 2014,14(31):6159-6163.
【11】程翼宇,钱忠直,张伯礼.创建以过程管控为核心的中药质量控制技术体系[J].中国中药杂志, 2017,42(1):1-5.
【12】周蓓.重庆道地药材山银花、青蒿和黄连主要有效成分与微量元素含量评价及相关性分析[D].重庆:西南大学, 2013.
【13】CHEN W Y, YANG Y C, FU K, et al. Progress in ICP-MS analysis of minerals and heavy metals in traditional medicine[J]. Frontiers in Pharmacology, 2022,13:891273.
【14】李丽敏,周如洁,曹帅,等.电感耦合等离子体质谱法在不同药典中的方法体系和在中药无机成分研究中的应用进展[J].理化检验-化学分册, 2021,57(7):665-672.
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