取2 mL水样，依次加入6 mg二水合柠檬酸三钠、1 mL 0.05 mol·L^-1四硼酸钠溶液和2 mL 1 g·L^-1 9-芴甲基氯甲酸酯（FMOC-Cl）溶液，在常温、250 W超声功率下衍生10 min。加入0.40 g氯化钠进行盐析，涡旋2 min，静置1 min，收集下层水相过0.45 μm滤膜，滤液用高效液相色谱法分析。以PAH C₁₈色谱柱作固定相，不同体积比乙腈和0.2%（体积分数）磷酸溶液的混合溶液作流动相进行梯度洗脱分离，荧光检测器检测。结果显示：草铵膦、草甘膦、氨甲基膦酸的质量浓度均在0.400~50.0 μg·L^-1内和其对应的衍生产物的峰面积呈线性关系，检出限（3.143s）分别为0.2，0.1，0.1 μg·L^-1。按照标准加入法进行回收试验，回收率为87.6%~100%，测定值的相对标准偏差（n=6）为5.7%~12%。方法用于实际水样的分析，检出结果均为阴性。

The 2 mL of water sample was taken, and 6 mg of trisodium citrate dihydrate, and 1 mL of 0.05 mol·L^-1 sodium tetraborate solution, and 2 mL of 1 g·L^-1 9-fluorene methyl chloroform (FMOC-Cl) solution were added sequentially. The mixed solution was reacted to derivatize targets at room temperature with ultrasonic power of 250 W for 10 min. Then 0.40 g of sodium chloride was added for salting out, and the mixture was vortexed for 2 min, and settled for 1 min. The lower water phase was collected, and passed through a 0.45 μm filter membrane, and the filtrate was analyzed by high performance liquid chromatography. PAH C₁₈ chromatographic column was used as the stationary phase, and the mixed solutions composed of acetonitrile and 0.2% (volume fraction) phosphoric acid solution at different volume ratios were used as the mobile phase for gradient elution separation, and fluorescence detector was used for detection. It was shown that linear relationships between values of the mass concentration of glufosinate, glyphosate, and aminomethylphosphonic acid and the peak area of their derivatization products were kept in the range of 0.400-50.0 μg·L^-1, with detection limits (3.143s) of 0.2, 0.1, 0.1 μg·L^-1, respectively. Test for recovery was made according to the standard addition method, giving recoveries in the range of 87.6%-100%, and RSDs (n=6) of the determined values ranged from 5.7% to 12%. The proposed method was used for the analysis of actual water samples, and the detection results were all negative.