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国产离子色谱-自研脉冲安培检测器测定饮料中常见糖类化合物的含量
          
Determination of Common Carbohydrate Compounds in Beverages by Domestic Ion Chromatography with Self-Designed Pulsed Amperometric Detector

摘    要
使用国产离子色谱-自研脉冲安培检测器测定饮料中常见的葡萄糖、果糖、乳糖、蔗糖和麦芽糖的含量。结果表明,5种糖类化合物的质量浓度在0.2~5.0 mg·L-1内与对应的峰面积呈线性关系,检出限(3S/N)分别为3.420,6.759,10.12,20.43,31.37 μg·L-1。按照标准加入法进行回收试验,回收率为94.1%~114%,测定值的相对标准偏差(n=5)均小于5.0%,说明方法符合相关检测标准要求。为考察国产仪器分析的准确性和评价主要模块的性能,与Thermo ICS 5000+型离子色谱安培检测系统和Dionex Ultimate 3000型液相色谱示差检测器系统进行比较,结果表明,三者的分析结果一致性良好,其中国产离子色谱-脉冲安培检测器系统的检出限比Thermo ICS 5000+离子色谱安培检测系统高3~4倍。除此之外,国产离子色谱仪各个模块性能稳定,可满足常规糖类化合物含量的测定。
标    签 国产离子色谱仪   自研脉冲安培检测器   饮料   糖类化合物   domestic ion chromatograph   self-designed pulse amperometric detector   beverage   carbohydrate compound  
 
Abstract
Glucose, fructose, lactose, sucrose and maltose commonly found in beverages were determined by domestic ion chromatography with self-designed pulsed amperometric detector. As shown by the results, linear relationship between values of the corresponding peak area and mass concentration of 5 carbohydrate compounds were found in the range of 0.2-5.0 mg·L-1, with detection limits (3S/N) of 3.420, 6.759, 10.12, 20.43, 31.37 μg·L-1, respectively. Test for recovery was made by the standard addition method, giving the results in the range of 94.1%-114%, with RSDs (n=5) of the determined values less than 5.0%, indicating that this method could meet the requirements of relevant testing standards. To investigate the accuracy of domestic instrument analysis and evaluate the performance of main modules, it was compared with Thermo ICS 5000+ion chromatograph amperometric detection system and Dionex Ultimate 3000 liquid chromatograph differential detector system, and it was shown that the analysis results were consistent, but the detection limit of domestic ion chromatography system with self-designed pulsed amperometric detector was 3 to 4 times higher than the imported instrument. The performance of each module and machine of domestic ion chromatography instrument was stable, and it could meet the requirements for the determination of conventional carbohydrate compounds.

中图分类号 O657.7+5   DOI 10.11973/lhjy-hx202312011

 
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收稿日期 2022/10/21

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备注郎蕾,硕士研究生,研究方向为离子色谱

引用该论文: LANG Lei,LIU Gelin,HOU Kunpeng,NI Tong,SHI Chaoou. Determination of Common Carbohydrate Compounds in Beverages by Domestic Ion Chromatography with Self-Designed Pulsed Amperometric Detector[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2023, 59(12): 1431~1436
郎蕾,刘格林,侯昆鹏,倪彤,施超欧. 国产离子色谱-自研脉冲安培检测器测定饮料中常见糖类化合物的含量[J]. 理化检验-化学分册, 2023, 59(12): 1431~1436


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参考文献
【1】佚名.碳水化合物-化学结构[J].淀粉与淀粉糖, 2010(2):36-44.
 
【2】ZHANG Z Q, KHAN N M, NUNEZ K M, et al. Complete monosaccharide analysis by high-performance anion-exchange chromatography with pulsed amperometric detection[J]. Analytical Chemistry, 2012,84(9):4104-4110.
 
【3】岳虹,赵贞,刘丽君,等.高效液相色谱法测定发酵乳饮料中果糖、葡萄糖、蔗糖、麦芽糖及乳糖含量[J].乳业科学与技术, 2017,40(2):23-26.
 
【4】樊宏,陈强.乳制品中乳糖直接比色测定方法探讨[J].中国卫生检验杂志, 2006,16(3):296-297.
 
【5】李雪梅,公卫景,秦国超,等.基于Fe3O4@CeO2类过氧化物模拟酶的葡萄糖比色传感检测[J].分析试验室, 2022,41(12):1545-1550.
 
【6】李甜,吴心茹,石京慧,等.基于纳米金银染放大的葡萄糖可视化检测[J].分析试验室, 2022,41(8):959-962.
 
【7】钟宁,侯彩云.三种乳糖检测方法的比较[J].食品科技, 2011,36(7):263-265.
 
【8】中华人民共和国卫生部,中国国家标准化管理委员会.食品中还原糖的测定:GB/T 5009.7-2003[S].北京:中国标准出版社, 2004.
 
【9】ZHANG J L, DAI X, SONG Z L, et al. One-pot enzyme- and indicator-free colorimetric sensing of glucose based on MnO2 nano-oxidizer[J]. Sensors and Actuators B:Chemical, 2020,304:127304.
 
【10】ZIELINSKI A A F, BRAGA C M, DEMIATE I M, et al. Development and optimization of a HPLC-RI method for the determination of major sugars in apple juice and evaluation of the effect of the ripening stage[J]. Food Science and Technology, 2014,34(1):38-43.
 
【11】SHANMUGAVELAN P, KIM S Y, KIM J B, et al. Evaluation of sugar content and composition in commonly consumed Korean vegetables, fruits, cereals, seed plants, and leaves by HPLC-ELSD[J]. Carbohydrate Research, 2013,380:112-117.
 
【12】MA C M, SUN Z, CHEN C B, et al. Simultaneous separation and determination of fructose, sorbitol, glucose and sucrose in fruits by HPLC-ELSD[J]. Food Chemistry, 2014,145:784-788.
 
【13】WU X D, JIANG W, LU J J, et al. Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry[J]. Food Chemistry, 2014,145:976-983.
 
【14】BAI W D, FANG X D, ZHAO W H, et al. Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography[J]. Journal of Food and Drug Analysis, 2015,23(4):645-651.
 
【15】HE J Z, XU Y Y, CHEN H B, et al. Extraction, structural characterization, and potential antioxidant activity of the polysaccharides from four seaweeds[J]. International Journal of Molecular Sciences, 2016,17(12):1988.
 
【16】DANIEL D, LOPES F S, DOS SANTOS V B, et al. Detection of coffee adulteration with soybean and corn by capillary electrophoresis-tandem mass spectrometry[J]. Food Chemistry, 2018,243:305-310.
 
【17】张欢欢,李疆,赵珊,等.毛细管区带电泳-间接紫外检测法快速测定食品中乳糖、蔗糖、葡萄糖和果糖[J].色谱, 2015,33(8):816-821.
 
【18】马海宁,华玉娟,屠春燕,等.毛细管电泳法分析藏红花植物细胞多糖中单糖组成[J].色谱, 2012,30(3):304-308.
 
【19】LV X Y, GUO Y X, ZHUANG Y P, et al. Optimization and validation of an extraction method and HPAEC-PAD for determination of residual sugar composition in L-lactic acid industrial fermentation broth with a high salt content[J]. Analytical Methods, 2015,7(21):9076-9083.
 
【20】WANG X, XU Y, LIAN Z N, et al. A one-step method for the simultaneous determination of five wood monosaccharides and the corresponding aldonic acids in fermentation broth using high-performance anion-exchange chromatography coupled with a pulsed amperometric detector[J]. Journal of Wood Chemistry and Technology, 2014,34(1):67-76.
 
【21】ZHANG Y, WU J R, NI Q H, et al. Multicomponent quantification of Astragalus residue fermentation liquor using ion chromatography-integrated pulsed amperometric detection[J]. Experimental and Therapeutic Medicine, 2017,14(2):1526-1530.
 
【22】彭友元,张清.基于还原氧化石墨烯-纳米铜的分子印迹电化学传感器测定葡萄糖[J].分析试验室, 2022,41(11):1297-1301.
 
【23】YOUNG C S. Evaporative light scattering detection methodology for carbohydrate analysis by HPLC[J]. Cereal Foods World, 2002,47:14-16.
 
【24】梁亚丽,张彦玲,何颖娜.糖类化合物分离分析方法进展[J].河北化工,2006,29(6):42-44.
 
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