提出了毛细管电泳联用电喷雾电离质谱法（CE-ESI-MS）测定吲哚-3-乙酸（IAA）、吲哚-3-丙酸（IPA）、萘乙酸（NAA）、6-苄基氨基嘌呤（BA）和脱落酸（ABA）等5种植物激素的含量。优化的分离部分的电泳条件和检测部分的电喷雾离子化质谱条件如下：电泳运行缓冲液为pH 6.0的15 mmol·L^-1的乙酸-乙酸铵缓冲液，有机改进剂为2%（体积分数）乙腈溶液，分离电压为22 kV，质谱分析以含0.11%（体积分数）乙酸的50%（体积分数）异丙醇溶液为鞘流液。在优化的分析条件下，上述5种植物激素在16 min内实现基线分离。5种植物激素的质量浓度在一定范围内与对应的峰面积呈线性关系，检出限（3S/N）为0.01~0.05 mg·L^-1。对同一混合标准溶液连续分析5次，5种植物激素峰面积的相对标准偏差均小于5.0%。按标准加入法进行回收试验，回收率为92.4%~114%。

A method was proposed for the determination of five plant hormones, including indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), naphthalene acetic acid (NAA), 6-benzylaminopurine (BA) and abscisic acid (ABA), by capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESI-MS). The optimized electrophoresis conditions for the separation and the electrospray ionization mass spectrometry conditions for the detection were as follows:15 mmol·L^-1 ammonium acetate-acetic acid buffer solution(pH 6.0) as electrophoresis running buffer solution, 2% (volume fraction) acetonitrile solution as organic modifier, the separation voltage of 22 kV, and 50%(volume fraction) isopropanol solution containing 0.11% (volume fraction) acetic acid as the sheath flow in mass spectrometry analysis.Under the optimal conditions, the above five plant hormones were separated at baseline within 16 min. Linear relationships between the corresponding peak area and mass concentration of the five plant hormones were found in definite ranges, with detection limits (3S/N) of 0.01-0.05 mg·L^-1. The RSDs of the peak area of the five plant hormones were less than 5.0% for 5 consecutive analysis of the same mixed standard solution. Test for recovery was made by the standard addition method, giving the results in the range of 92.4%-114%.