取一定量牡蛎样品的匀浆,用水和乙腈提取;分取部分乙腈提取液,按给定条件用2,4-二硝基氟苯对溶液中牛磺酸进行柱前衍生。将反应后的溶液用乙腈稀释10倍,分取20 μL进行高效液相色谱分析。用Ultimate C18色谱柱为固定相,以不同体积比混合的乙腈和0.2%(φ)三乙胺(用磷酸调节其酸度至pH 4.0)混合液作为流动相进行梯度淋洗,所用检测波长为360 nm。经衍生后牛磺酸浓度在4.68×10-10~4.25×10-5mol·L-1范围内与其相应的峰面积呈线性关系。方法的检出限(3S/N)为5.5×10-11mol·L-1。用标准加入法做回收试验,测得平均回收率为97.4%,测定值的平均相对标准偏差(n=6)为2.1%。

Definite amount of homogenized pulp of oyster was taken as sample, which was extracted successively with water and acetonitrile. An aliquot of the acetonitrile extract was taken and taurine in the extract was pre-column derivatized with 2,4-dinitrofluorobenzene under prescribed conditions. The reaction solution was diluted 10 times with acetonitrile. An aliquot of 20 μL of the solution was used for HPLC analysis. In the chromatographic separation, Ultimate C18 column was used as stationary phase, and mixtures of acetonitrile and 0.2% (φ) triethylamine-H2O solution (preadjusted to pH 4.0 with H3PO4) mixed in various ratios were used as mobile phase in the gradient elution. Detection wavelength used was 360 nm. Linear relationship between values of peak area and concentration of taurine (determined as its derivate) was kept in the range of 4.68×10-10 to 4.25×10-5mol·L-1, with detection limit (3S/N) of 5.5×10-11mol·L-1. Recovery was tested by standard addition method, giving values of average recovery of 97.4%, and RSD (n=6) of 2.1%.