提出了高效液相色谱法同时测定可食性包装材料中3种工业酸性红染料(酸性红14、酸性红35和酸性红73)的方法。样品经超声波辅助提取,以C18色谱柱(250 mm×4.6 mm,5 μm)为固定相,用10 mmol·L-1乙酸铵溶液和甲醇以不同比例混合的溶液为流动相进行梯度洗脱,用紫外检测器测定。3种酸性红染料的质量浓度均在5.0~80 mg·L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0.08~0.15 mg·kg-1之间。在10.0,20.0,30.0 mg·L-1等3个浓度水平进行加标回收试验,回收率在89.4%~97.1%之间,测定值的相对标准偏差(n=6)在0.18%~1.6%之间。

A method of HPLC for simultaneous measurement of 3 industrial acidic red dyes (Acid Red 14, Acid Red 35 and Acid Red 73) in edible packaging was proposed. Ultrasonic extraction was used as sample preparation. The stationary phase was a C18 column (250 mm×4.6 mm, 5 μm) with mixtures of 10 mmol·L-1 ammonium acetate and methanol at different ratios as mobile phase for gradient elution, UV detector was used. Linear relationships between peak area and mass concentration of the 3 acidic red dyes were in the same range of 5.0-80 mg·L-1, with detection limits (3S/N) in the range of 0.08-0.15 mg·kg-1. Recovery measured by standard addition method at concentration of 10.0, 20.0, 30.0 mg·L-1, and RSD′s (n=6) were in the ranges of 89.4%-97.1% and 0.18%-1.6%, respectively.