提出了毛细管电泳快速测定淀粉中可乐定含量的方法。以pH 2.2的磷酸-25 mmol·L-1磷酸二氢钠缓冲溶液为电解质溶液,分离电压20 kV,于214 nm波长处进行紫外检测。在优化的试验条件下,可乐定的质量浓度在0.3~200 mg·L-1范围内与其对应的校正峰面积呈线性关系。检出限(3S/N)为0.1 mg·L-1,测定下限(10S/N)为0.3 mg·L-1。采用该方法对淀粉样品中可乐定含量进行测定,所得加标回收率在97.1%~98.4%之间,相对标准偏差(n=5)小于2.0%。

A method for the rapid determination of clonidine in starch by capillary electrophoresis (CE) was proposed. A mixture of phosphoric acid and 25 mmol·L-1 sodium dihydrogen phosphate solution of pH 2.2 was used as background electrolyte, and separation voltage of 20 kV was applied. UV-detection at the wavelength of 214 nm was adopted. Under the optimized conditions, linear relationship between values of corrected peak area and mass concentration of clonidine was obtained in the range of 0.3 to 200 mg·L-1, with detection limit (3S/N) and lower limit of determination (10S/N) of 0.1 mg·L-1and 0.3 mg·L-1 respectively. Recovery and precision of the method were tested by standard addition method, the results of recovery found were in the range of 97.1 %-98.4 % and RSD′s (n=5) were less than 2.0%.