1-羟基芘与牛血清白蛋白的相互作用
Interaction between 1-Hydroxypyrene with Bovine Serum Albumin
摘 要
采用荧光光谱法和紫外-可见分光光度法研究了1-羟基芘与牛血清白蛋白之间的相互作用。结果表明:1-羟基芘对牛血清白蛋白有较强的荧光猝灭作用。根据由Stern-Volmer方程得到的荧光猝灭常数,可判断由于与1-羟基芘反应而导致牛血清白蛋白的荧光猝灭属于静态猝灭。采用位点结合模型公式和Frster非辐射能量转移理论计算了结合常数、结合位点数、结合距离。从计算得到的热力学参数焓变ΔH和熵变ΔS,推断了1-羟基芘与牛血清白蛋白反应的作用力为氢键和范德华力。
牛血清白蛋白
相互作用
荧光光谱法
分光光度法
1-Hydroxypyrene
Bovine serum albumin
Interaction
Fluorospectrometry
Spectrophotometry
Abstract
Mechanism of the interaction between 1-hydroxypyrene (1-HP) and bovine serum albumin (BSA) was studied by fluorospectrometry and UV-VIS spectrophotometry. It was found that significant quenching of fluorescence of BSA appeared by its reaction with 1-HP. As judged from the fluorescence quenching constants obtained by Stern-Volmer equation, fluorescence quenching of BSA by 1-HP was attributed to static quenching. The binding constant, number of binding sites and binding distance were found by applying the equation of site-binding model and Frster′s nonradiative energy transfer theory. Values of thermodynamic parameters ΔH and ΔS were calculated and based on these results, the binding force of the reaction was due to hydrogen bond and Vander Waals force.
中图分类号 O657.32
所属栏目
基金项目 湖南省自然科学基金资助项目(03JJY3030);常州市自然科学基金资助项目(CS2008209)
收稿日期 2010/4/3
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备注欧阳运富(1971-),男,湖南宁远人,副主任技师,硕士,主要从事环境中有毒有害物质的检测及分子机理的研究。
引用该论文: OUYANG YUN-fu,WANG Yong-sheng,LI Gui-ying. Interaction between 1-Hydroxypyrene with Bovine Serum Albumin[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2011, 47(3): 253～256
欧阳运富,王永生,李贵英. 1-羟基芘与牛血清白蛋白的相互作用[J]. 理化检验－化学分册, 2011, 47(3): 253～256
被引情况:
【1】赵婷婷,毕淑云,王瑜,王天娇, "荧光光谱法研究山姜素与牛血清白蛋白的相互作用机理",理化检验－化学分册 50, 944-948(2014)
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参考文献
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【7】SHAIKH S M T, SEETHARAMAPPA J, KADAGAL P B, et al. In vitro study on the binding of anti-coagulant vitamin to bovine serum albumin and the influence of toxic ions and common ions on binding［J］. Int J Biol Macromol, 2007,41:81-86.
【8】欧阳运富,王永生,唐宏兵.聚乙二醇辛基苯基醚胶束增敏荧光分光光度法测定人尿中痕量1-羟基芘［J］.理化检验-化学分册, 2009,45(10):1194-1196.
【2】VIAU C, DIAKIT A, RUZGYT A, et al. Is 1-hydroxypyrene a reliable bioindicator of measured dietary polycyclic aromatic hydrocarbon under normal conditions［J］. J Chromatogr B, 2002,778:165-177.
【3】KANDAGAL P B, ASHOKA S, SEETHARAMAPPA J, et al. Study of the interaction between doxepin and human serum albumin by spectroscopic methods［J］. J Photochem Photobiol A, 2006,179:161-166.
【4】CUI Feng-ling, FAN Jing, LI Jian-ping, et al. Interactions between 1-benzoyl-4-p-chlorophenyl thiosemicarbazide and serum albumin: investigation by fluorescence spectroscopy［J］. Bioorg Med Chem, 2004,12:151-157.
【5】HU Yan-jun, LIU Wei, LIU Yi, et al. Fluorometric investigation of the interaction between methylene blue and human serum albumin［J］. J Pharm Biomed Anal, 2005,39:740-745.
【6】HU Yan-jun, LIU Yi, SHEN Xue-song, et al. Studies on the interaction between 1-hexylcarbamoyl-5-fluorouracil and bovine serum albumin［J］. J Mol Struct, 2005,738:143-147.
【7】SHAIKH S M T, SEETHARAMAPPA J, KADAGAL P B, et al. In vitro study on the binding of anti-coagulant vitamin to bovine serum albumin and the influence of toxic ions and common ions on binding［J］. Int J Biol Macromol, 2007,41:81-86.
【8】欧阳运富,王永生,唐宏兵.聚乙二醇辛基苯基醚胶束增敏荧光分光光度法测定人尿中痕量1-羟基芘［J］.理化检验-化学分册, 2009,45(10):1194-1196.
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