提出了高效液相色谱法测定烟草中水溶性糖含量的方法。烟草样品经0.01 mol·L-1氢氧化钠溶液振荡提取60 min,以Metacarb 67C色谱柱(300 mm×6.5 mm)为固定相,纯水为流动相等梯度洗脱,用示差折光检测器检测。烟草中主要的水溶性糖均在12 min内达到基线分离,果糖、葡糖糖和蔗糖的检出限(3S/N)分别为0.60,0.71,0.39 mg·L-1。方法加标回收率在98.1%~99.2%之间,相对标准偏差(n=8)在0.97%~1.6%之间。

A method of HPLC for the determination of water soluble saccharides in tobacco was proposed. The sample was extracted vortically with 0.01 mol·L-1 NaOH solution for 60 min, and the extract obtained was separated by isogradient elution with pure water on Metacarb 67C column (300 mm×6.5 mm), and determined with differential refraction detector. The main water soluble saccharides in tobacco were successfully separated within 12 min, and detection limits (3S/N) found were 0.60 mg·L-1 for fructose, 0.71 mg·L-1 for glucose and 0.39 mg·L-1 for sucrose. Values of recovery and RSD′s (n=8) of the 3 water soluble saccharides found were in ranges of 98.1%-99.2% and 0.97%-1.6% respectively.