用石油醚作溶剂在索氏提取器中分别提取东北小鲵体内组织(皮、肌肉及内脏)中的粗脂肪油,所得萃取液分别用饱和氯化钠溶液及纯水洗涤后加入无水硫酸钠脱水过夜。用旋转蒸发除去萃取液中有机溶剂,于是获得3份油状液体分别为从小鲵的皮、肌肉及内脏提取的脂肪酸。从中分取0.20 mL于氢氧化钾溶液中加甲醇做甲酯化处理,用乙醚-正己烷(2+1)混合溶剂1.0 mL萃取所生成的脂肪酸甲酯。从上层清液中分取0.4 μL试液进行气相色谱-质谱分析。在肌肉提取液中鉴定出21种脂肪酸,以油酸和亚油酸为主,其中不饱和脂肪酸占66.26%;在皮提取液中鉴定出8种脂肪酸,以油酸和亚麻酸为主,其中不饱和脂肪酸占68.79%;在内脏提取液中鉴定出9种脂肪酸,以油酸、亚油酸和棕榈酸为主,其中不饱和脂肪酸占65.64%。

Crude fatty oil of various tissues (skin, muscle and viscera) of freeze-dried Hynobius leechii were extracted separately with petroleum ether in Soxhlet′s extractor. The extracts were washed separately with saturated NaCl solution and distilled water, and dehydrated overnight with anhydrous Na2SO4. The solvent in the extract was removed by rotary evaporation, and 3 portions of oil separated from the tissues of Hynobius leechii were obtained. An aliquot of 0.20 mL was taken and esterified with methyl alcohol in KOH solution and extracted with 1.0 mL of mixture of ethyl ether and n-hexane (2+1), 0.4 mL of the supernatant was taken and analyzed by GC-MS. In portion of oil from the muscle, 21 fatty acids were identified, in which oleic acid and linoleic acid were the main components, and the total of unsaturated fatty acids amounted to 66.26%. In the oil from the skin, 8 fatty acids were identified with oleic acid and linolenic acid as the main components, and with total amount of unsaturated fatty acids achieved to 68.79%. In the oil from viscera, 9 fatty acids were identified with oleic acid, linoleic acid and palmitic acid as main components, and with the total of unsaturated fatty acids amounted to 65.64%.