Identification of Raw and Ripe Pu-Er Tea by HPLC-MS/MS with Pattern Recognition
摘 要
采用高效液相色谱-质谱联用技术及高效液相色谱法对生熟普洱茶中的主要成分进行定性和定量分析。鉴定出普洱茶水溶液中8种主要成分,分别为没食子酸(GA)、没食子酸儿茶素(GC)、表没食子酸儿茶素(EGC)、儿茶素(C)、咖啡因(CAF)、表儿茶素(EC)、表没食子酸儿茶素没食子酸酯(EGCG)和表儿茶素没食子酸酯(ECG)。以这8种成分的含量为指标,对普洱生茶和熟茶各20批进行主成分分析、聚类分析和判别分析,能准确地区分普洱生茶与熟茶。
Abstract
HPLC-MS/MS and HPLC were applied to the qualitative and quantitative analysis of raw and ripe Pu-Er tea. Eight main components were identified; they were gallic acid (GA), gallocatechin (GC), epigallocatechin (EGC), catechin (C), caffeine (CAF), epicatechin (EC), epigallocatechin gallate (EGCG) and epicatechin gallate (ECG). The contents were used as characteristic indexes for the identification of 20 batches each of the raw and ripe tea by pattern recognition techniques, including principal component analysis (PCA), clustering analysis (CA) and linear discriminant analysis (LDA). The proposed method was useful for distinguishing between raw and ripe Pu-Er tea.
中图分类号 O657.63
所属栏目
基金项目
收稿日期 2010/11/6
修改稿日期
网络出版日期
作者单位点击查看
联系人作者倪倩(niqian87@163.com)
备注倪倩(1987-),女,江苏淮安人,硕士,主要研究方向为中药质量评价与研究。
引用该论文: NI Qian,TONG Ling,GAO Jun,LI Wen-bo,WANG Qiang. Identification of Raw and Ripe Pu-Er Tea by HPLC-MS/MS with Pattern Recognition[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(2): 171~174
倪倩,佟玲,高钧,李文博,王强. 高效液相色谱-质谱法及模式识别法用于鉴别普洱生茶与熟茶[J]. 理化检验-化学分册, 2012, 48(2): 171~174
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】陈宗懋.中国茶叶大辞典[M].北京:中国轻工业出版社, 2000.
【2】杨崇仁,陈可可,张颖君.茶叶的分类与普洱茶的定义[J].茶叶科学技术, 2006(2):37-39.
【3】VALERA P, PABLOS F, GONZALEZ A G. Characterization of tea samples by their chemical composition using discriminant analysis[J]. Talanta, 2003,43:415-419.
【4】FERNANDEZ P L, MARTIN M J, GONZALEZ A G, et al. HPLC determination of catechins and caffeine in tea[J]. Analyst, 2000,125:421-425.
【5】BUDINOVA G, VLACIL D, MESTEK O, et al. Application of infrared spectroscopy to the assessment of authenticity of tea[J]. Talanta, 1998,47:255-260.
【6】FERNANDEZ-CACERES P L, MARTIN M J, PABLOS F, et al. Differentiation of tea (camellia sinensis) varieties and their geographical origin according to their metal content[J]. Agric Food Chem, 2001,49:4775-4779.
【7】MOREDA-PINEIRO A, FISHER A, HILL S J. The classification of tea according to region of origin using pattern recognition techniques and trace metal data[J]. Journal of Food Composition and Analysis, 2003,16:195-211.
【8】RAFAEL C C, GIMENEZ M C L. Determination of tea components with antioxidant activity[J]. Agric Food Chem, 2003,51:4427-4435.
【9】BEEBE K R, PELL R J, SEASHOLTZ M B. Chemometrics: A Practical Guide[M]. USA: Wiley, 2008:56.
【2】杨崇仁,陈可可,张颖君.茶叶的分类与普洱茶的定义[J].茶叶科学技术, 2006(2):37-39.
【3】VALERA P, PABLOS F, GONZALEZ A G. Characterization of tea samples by their chemical composition using discriminant analysis[J]. Talanta, 2003,43:415-419.
【4】FERNANDEZ P L, MARTIN M J, GONZALEZ A G, et al. HPLC determination of catechins and caffeine in tea[J]. Analyst, 2000,125:421-425.
【5】BUDINOVA G, VLACIL D, MESTEK O, et al. Application of infrared spectroscopy to the assessment of authenticity of tea[J]. Talanta, 1998,47:255-260.
【6】FERNANDEZ-CACERES P L, MARTIN M J, PABLOS F, et al. Differentiation of tea (camellia sinensis) varieties and their geographical origin according to their metal content[J]. Agric Food Chem, 2001,49:4775-4779.
【7】MOREDA-PINEIRO A, FISHER A, HILL S J. The classification of tea according to region of origin using pattern recognition techniques and trace metal data[J]. Journal of Food Composition and Analysis, 2003,16:195-211.
【8】RAFAEL C C, GIMENEZ M C L. Determination of tea components with antioxidant activity[J]. Agric Food Chem, 2003,51:4427-4435.
【9】BEEBE K R, PELL R J, SEASHOLTZ M B. Chemometrics: A Practical Guide[M]. USA: Wiley, 2008:56.
相关信息