Spectrophotometric Determination of Sibutramine Hydrochloride with Bromophenol Blue as Color Reagent
摘 要
在pH 3.70的B-R缓冲溶液中,盐酸西布曲明与溴酚蓝以摩尔比1比2反应形成具有正吸收和负吸收的离子缔合物,最大正吸收波长为436 nm,最大负吸收波长为590 nm,线性范围分别在3.4×10-6mol·L-1(正吸收)、3.8×10-6mol·L-1(负吸收)以内,表观摩尔吸光率为1.83×105L· mol-1·cm-1(正吸收)、5.51×104L· mol-1·cm-1(负吸收),据此建立了测定盐酸西布曲明含量的分光光度法。方法用于合成样品及尿样中盐酸西布曲明的测定,回收率在96.8%~102.5%,相对标准偏差(n=6)在0.9%~1.7%之间。
Abstract
In a B-R buffer solution of pH 3.70, an ionic association complex, with molar ratio of 1 to 2 was formed by the reaction of sibutramine hydrochloride with bromophenol blue, having the positive absorption maxmum at the wavelength of 436 nm and negative absorption maximum at 590 nm. Ranges of linearity found at the 2 absorption maxima were within 3.4×10-6mol·L-1 and 3.8×10-6mol·L-1, with their values of apparent molar absorptivity of 1.83×105L· mol-1·cm-1 and 5.51×104L·mol-1·cm-1 respectively. Based on these facts, a method of spectrophotometric determination of sibutramine hydrochloride was proposed. The present method was applied to the determination of sibutramine hydrochloride in simulated samples and urine samples, giving values of recovery and RSD′s (n=6) in the ranges of 96.8%-102.5% and 0.9%-1.7% respectively.
中图分类号 O657.32
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基金项目 重庆市教育委员会科学技术项目(No:KJ101314)资助
收稿日期 2011/1/7
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备注谢兵(1964-),男,重庆人,高级实验师,理学学士,主要从事化学教育与化学应用的研究工作。
引用该论文: XIE Bing,PANG Xiang-dong,TANG Wei,TAN Rong,QIN Zong-hui. Spectrophotometric Determination of Sibutramine Hydrochloride with Bromophenol Blue as Color Reagent[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(2): 229~232
谢兵,庞向东,汤伟,谭蓉,秦宗会. 溴酚蓝分光光度法测定盐酸西布曲明[J]. 理化检验-化学分册, 2012, 48(2): 229~232
被引情况:
【1】李杰,许卓妮,于瑞祥,李永利,陈鹰,王虎, "超高效液相色谱-串联质谱法测定减肥类保健品中的西布曲明",理化检验-化学分册 51, 995-998(2015)
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【2】沈敬山,李剑峰,毛海舫,等.盐酸西布曲明的合成[J].中国医药工业杂志, 2001,32(8):337-339.
【3】左晓春,笪宏远.新一代减肥药-西布曲明[J].中国临床药理学杂志, 2000,16(2):155-156.
【4】秦宗会,谭蓉.柠檬酸与乙酸酐混合溶液中分光光度法测定盐酸西布曲明[J].理化检验-化学分册, 2007,43(12):1045-1047.
【5】秦宗会,谭蓉,蒲利军,等.刚果红褪色光度法测定盐酸西布曲明及作用机理研究[J].分析化学, 2006,34(3):403-406.
【6】RADHAKRISHNA T, LAKSHMI N C H, SREENIVAS R D, et al. LC method for the determination of as say and purity of sibutramine hydrochloride and its enantiomers by chiral chromatography[J]. Journal of Pharmaceutical and Biomedical Analysis, 2000,22(4):627-639.
【7】曹文丁,余佳文,官柳,等.高效液相色谱法测定曲美胶囊中盐酸西布曲明的含量[J].中国药房, 2004,15(4):240-241.
【8】高素英,陈雪帆.盐酸西布曲明片的溶出度测定[J].药学实践杂志, 2003,21(6):371-373.
【9】黄芳,陈华,夏之宁.西布曲明对映体的毛细管电泳分离与定量分析方法研究[J].药物分析杂志, 2004,24(2):113-116.
【10】DING Li, HAO Xin-yu, HUANG Xin, et al. Simultaneous determination of sibutramine and its n-desmethyl metabolites in human plasma by liquid chromatography electrometry ionization-mass spectrometry method and clinical applications[J]. Anal Chim Acta, 2003,492(1/2):241-248.
【11】秦宗会,刘艳,谭蓉,等.衍生荧光法测定盐酸西布曲明[J].分析试验室, 2006,25(4):9-12.
【12】周尚,杨季冬.卤代荧光素与西布曲明相互作用的共振散射光谱特征及其分析应用[J].分析测试学报, 2008,27(10):1058-1061.
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