角膜接触镜护理液样品以乙酸乙酯超声提取,提取液经离心后供气相色谱测定。采用DB-Wax毛细管柱(30 m×0.32 mm,0.5 μm)分离样品,氢火焰离子化检测器检测,内标法定量。薄荷脑线性范围为3.74~187.2 mg·L-1,方法的检出限(3S/N)为0.15 mg·L-1。方法用于护理液中薄荷脑的测定,回收率在98.3%~99.3%之间;相对标准偏差(n=6)在0.72%~1.95%之间。

Sample of cornea contact lens solution was ultrasonically extracted with ethyl acetate, centrifuged and the supernatant was used for GC determination. DB-Wax capillary column (30 m×0.32 mm, 0.5 μm) was used for separation. FID and internal standard method were adopted in the determination. Linearity range of menthol found was between 3.74 and 187.2 mg·L-1, with detection limit (3S/N) of 0.15 mg·L-1. The proposed method was applied to the determination of menthol in cornea contact lens solution, giving values of recovery and RSD′s (n=6) in the ranges of 98.3%-99.3% and 0.72%-1.95% respectively.