Simultaneous Differential Pulse Voltammetric Determination of Dopamine and Uric Acid with Polycalconcarboxylic Acid Modified Glassy Carbon Electrode
摘 要
采用电化学方法将钙羧酸(CCA)聚合修饰在玻碳电极(GCE)表面制备了聚钙羧酸指示剂修饰玻碳电极(PCCA/GCE),并用循环伏安法和交流阻抗法研究了电极的电化学性能。结果表明:在pH 6.0的磷酸盐缓冲溶液中,多巴胺(DA)和尿酸(UA)在聚钙羧酸修饰电极上的氧化峰得以分开,峰电位差为0.14 V,据此提出了聚钙羧酸修饰电极差分脉冲伏安法同时测定多巴胺和尿酸的方法。DA和UA的浓度分别在5.0~43.8 μmol·L-1和5.0~50.0 μmol·L-1范围内与其氧化峰电流呈线性关系,检出限(3S/N)分别为0.2 μmol·L-1和0.5 μmol·L-1。方法可用于多巴胺注射液样品中DA和UA的测定,测定值的相对标准偏差(n=5)依次为2.43%和2.35%。
Abstract
The modified electrode (PCCA/GCE) was prepared by electrochemical polymerization of calconcarboxylic acid (CCA) on the surface of glassy carbon electrode (GCE). The electrochemical property of the PCCA/GCE was studied by cyclic voltammetry and AC impedance method. It was found that in a PBS of pH 6.0, the oxidation peaks of dopamine (DA) and uric acid (UA) were completely separated, and the potential difference of the oxidation peaks attained to a value of 0.14 V. Based on these findings, a selective method for simultaneous determination of DA and UA by differential pulse voltammetry was proposed. Linear relationships between values of oxidation peak current and concentration of DA and UA were obtained in the ranges of 5.0-43.8 μmol·L-1 and 5.0-50.0 μmol·L-1, with their detection limits of 0.2 μmol·L-1 and 0.5 μmol·L-1 respectively. The proposed method has been used in simultaneous determination of DA and UA in DA injections, giving values of RSD′s (n=5) of 2.43% for DA and 2.35% for UA.
中图分类号 O657.1
所属栏目 试验与研究
基金项目 上海市自然科学基金(09ZR1423500);上海市教委创新项目(09YZ161)资助
收稿日期 2011/7/8
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备注黄文华(1968-),男,河南永城人,副教授,主要研究方向为电分析化学。
引用该论文: HUANG Wen-hua,HOU Bao-qin,ZHANG Lei. Simultaneous Differential Pulse Voltammetric Determination of Dopamine and Uric Acid with Polycalconcarboxylic Acid Modified Glassy Carbon Electrode[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(6): 638~642
黄文华,侯宝芹,张雷. 聚钙羧酸修饰玻碳电极差分脉冲伏安法同时测定多巴胺和尿酸[J]. 理化检验-化学分册, 2012, 48(6): 638~642
被引情况:
【1】李春哲,潘虹,陈立波,赵兰坡, "微分脉冲阳极溶出伏安法同时测定两种抗病毒药物",理化检验-化学分册 49, 1103-1106(2013)
【2】张莹,张雷, "聚2,6-二氨基-4,5,6,7-四氢苯并噻唑修饰玻碳电极伏安法测定多巴胺和尿酸",理化检验-化学分册 50, 797-804(2014)
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参考文献
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【2】OYAMA N, ANSON F C. Catalysis of electrode processes by multiply-charged metal complexes electrostatically bound to polyelectrolyte coatings on graphite electrodes, and the use of polymer-coated rotating disk electrodes in diagnosing kinetic and conduction mechanisms[J]. Anal Chem, 1980,52:1192-1198.
【3】ZARE H R, MEMARZADEH F, ARDAKANI M M, et al. Norepinephrine modified glassy carbon electrode for the simultaneous determination of ascorbic acid and uric acid[J]. Electrochim Acta, 2005,50:3495-3502.
【4】邹冈.基础神经药理学[M].2版.北京:科学出版社, 1999:203.
【5】DRYHURST G. Electrochemistry of biologicalmolecules[M]. New York: Academic Press, 1977.
【6】YANG Su-ling, LI Gang, YANG Ran, et al. Simultaneous voltammetric detection of dopamine and uric acid in the presence of high concentration of ascorbic acid using multi-walled carbon nanotubes with methylene blue composite film-modified electrode[J]. J Solid State Electrochem, 2011,15:1909-1918.
【7】SUKRIYE U, ZEKERYA D. Cu nanoparticles incorporated polypyrrole modified GCE for sensitive simultaneous determination of dopamine and uric acid[J]. Talanta, 2010,80:1461-1466.
【8】WANG Po, LI Yong-xin, HUANG Xue, et al. Fabrication of layer-by-layer modified multilayer films containing choline and gold nanoparticles and its sensing application for electrochemical determination of dopamine and uric acid[J]. Talanta, 2007,73:431-437.
【9】HARISH S, MATHIYARASU J, PHANI K L N, et al. PEDOT/palladium composite material: synthesis, characterization and application to simultaneous determination of dopamine and uric acid[J]. J Appl Electrochem, 2008,38:1583-1588.
【10】LIU Ai-lin, ZHANG Shao-bo, CHEN Wei, et al. Simultaneous voltammetric determi-nation of norepinephrine, ascorbic acid and uric acid on polycalconcarboxylic acid modified glassy carbon electrode[J]. Biosens Bioelectron, 2008,23:1488-1495.
【11】FINKLEA H O, SNIDER D A, FEDYK J, et al. Characterization of octadecanethiol-coated gold electrodes as microarray electrodes by cyclic voltammetry and AC impedance spectroscopy[J]. Langmuir, 1993,9:3660-3667.
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